Launen L, Pinto L, Wiebe C, Kiehlmann E, Moore M
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada
Can J Microbiol. 1995 Jun;41(6):477-88. doi: 10.1139/m95-064.
The purpose of this study was to determine the ability of nonbasidiomycete soil fungi to oxidize pyrene (four rings) and benzo[a]pyrene (BaP) (five rings). Fungi were isolated from five different soils in which the polycyclic aromatic hydrocarbon content ranged from 0.8 to 80 micrograms/g dry soil. Approximately 50% of the isolates in all sites were able to oxidize pyrene. The pyrene-oxidizing species belonged to all fungal divisions except basidiomycetes. The most common were Penicillium spp. of the subgenus Furcatum and these dominated the more contaminated soils. Penicillium janthinellum and Syncephalastrum racemosum exhibited the most rapid rates of pyrene oxidation. The major pyrene metabolites were identified by proton NMR and mass spectrometry as 1-pyrenol, 1,6- and 1,8-pyrenediol, and the 1,6-and 1,8-pyrenequinones. A high correlation was found between the ability to oxidize pyrene and BaP. As with pyrene, approximately 50% of the fungal isolates tested oxidized BaP to 9-hydroxy-BaP. Eighty percent of the pyrene-oxidizing strains were also able to metabolize BaP.
本研究的目的是确定非担子菌土壤真菌氧化芘（四环）和苯并[a]芘（BaP，五环）的能力。从五种不同的土壤中分离出真菌，这些土壤中多环芳烃的含量在0.8至80微克/克干土之间。所有采样点约50%的分离菌株能够氧化芘。除担子菌外，芘氧化菌属于所有真菌门类。最常见的是叉状亚属的青霉属物种，它们在污染更严重的土壤中占主导地位。淡紫青霉和总状共头霉表现出最快的芘氧化速率。芘的主要代谢产物经质子核磁共振和质谱鉴定为1-芘醇、1,6-和1,8-芘二醇以及1,6-和1,8-芘醌。发现氧化芘和BaP的能力之间存在高度相关性。与芘一样，约50%的受试真菌分离株将BaP氧化为9-羟基-BaP。80%的芘氧化菌株也能够代谢BaP。
