Adachi T, Yamada H, Futenma A, Kato K, Hirano K
Department of Pharmaceutics, Gifu Pharmaceutical University.
J Biochem. 1995 Mar;117(3):586-90. doi: 10.1093/oxfordjournals.jbchem.a124748.
Extracellular-superoxide dismutase [EC 1.15.1.1] (EC-SOD) is a secretory, tetrameric glycoprotein. A prominent feature of EC-SOD is its affinity for heparin. This enzyme in serum is heterogeneous with regard to heparin-affinity and can be divided into five fractions (I) to (V) by heparin-HPLC, whereas fibroblast-secreted EC-SOD consists mainly of form (V). An intravenous injection of 50 i.u. of heparin/kg body weight into two healthy volunteers led to an immediate rise of serum EC-SOD level by 2.4-2.8-fold. Only form (V), which was a minor component in pre-heparin serum, was increased by the intravenous injection. The half-life of serum EC-SOD after the prompt rise was about 90 min. The in vivo experiment using rats and an in vitro experiment strongly suggested the EC-SOD released into the plasma reconstituted the interaction with glycocalyx on the vascular endothelial cell surface in accordance with the elimination of heparin from the vascular system.
细胞外超氧化物歧化酶EC 1.15.1.1是一种分泌型四聚体糖蛋白。EC-SOD的一个显著特征是它对肝素具有亲和力。血清中的这种酶在肝素亲和力方面具有异质性，通过肝素高效液相色谱法可分为五个组分(I)至(V)，而成纤维细胞分泌的EC-SOD主要由(V)型组成。给两名健康志愿者静脉注射50国际单位/千克体重的肝素后，血清EC-SOD水平立即升高2.4至2.8倍。静脉注射仅使肝素前血清中的次要组分(V)型增加。血清EC-SOD迅速升高后的半衰期约为90分钟。使用大鼠进行的体内实验和体外实验有力地表明，随着肝素从血管系统中清除，释放到血浆中的EC-SOD会重新与血管内皮细胞表面的糖萼相互作用。
