Abed Y, Bollet C, De Micco P
Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hôpital Salvator, Marseille, France.
J Med Microbiol. 1995 Aug;43(2):156-8. doi: 10.1099/00222615-43-2-156.
Amplification of the region separating the genes coding for the two rRNA species 16S and 23S was performed with 56 strains of several mycobacterial species, including 21 clinical isolates of Mycobacterium kansasii and the M. kansasii type strain ATCC 12478. On the basis of PCR product profiles, the previously suggested heterogeneity of M. kansasii species was confirmed. Three subgroups were identified; members of the first subgroup showed the same PCR profile as the reference strain. Different profiles were obtained for the two other subgroups. Amplification of the 16S-23S spacer is rapid and simple and, consequently, may be a helpful tool for identification and characterisation of M. kansasii isolates in epidemiological analysis.
对包括21株堪萨斯分枝杆菌临床分离株和堪萨斯分枝杆菌标准菌株ATCC 12478在内的几种分枝杆菌属的56个菌株，进行了编码两种核糖体RNA（16S和23S）的基因之间区域的扩增。根据PCR产物谱，证实了先前提出的堪萨斯分枝杆菌种的异质性。鉴定出三个亚组；第一亚组的成员显示出与参考菌株相同的PCR谱。另外两个亚组获得了不同的谱。16S - 23S间隔区的扩增快速且简单，因此，在流行病学分析中可能是鉴定和表征堪萨斯分枝杆菌分离株的有用工具。
