Cook C B, Nair R, Herminghuysen D, Gatchair-Rose A, Rao J, Bagby G J, Prasad C
Department of Medicine, Louisiana State University Medical Center, New Orleans, USA.
Life Sci. 1995;57(9):839-45. doi: 10.1016/0024-3205(95)02016-c.
Adipose tissue lipoprotein lipase (LPL) is a key enzyme responsible for the clearance of circulating triglycerides and has been linked to certain pathologic states such as obesity. In order to investigate whether an animal's endogenous fat-preference is associated with differences in adipose tissue LPL, we measured enzyme activity in epididymal fat from high- and low-fat preferring rats. Utilizing a 24h ad libitum feeding paradigm, four groups of outbred adult male Sprague-Dawley rats were screened separately for their macronutrient preferences. Animals exhibiting high- or low-fat preferences were identified and placed back on standard chow. LPL activity was measured in epididymal fat under chow fed or fasted conditions. Epididymal fat LPL activity was significantly less in the high-fat-preferring animals relative to the low-fat-preferring, in both the standard chow-fed state (p = 0.014) and fasted (p = 0.0007) state. LPL activity in heart ventricle and brown adipose tissue was also measured from the same animals. Activity in heart ventricle and brown adipose tissue was significantly lower in the high-fat-preferring group as compared with the low-fat-preferring only following a 24h fast (p = 0.0012 for heart and p = 0.0085 for brown adipose, high- versus low-fat preferring). The data indicate that differences in tissue LPL activity exist between animals with inherent differences in fat preference. Future comparative studies between the two groups of fat-preferring animals could lead to important clues to the regulation of the LPL.
脂肪组织脂蛋白脂肪酶（LPL）是负责清除循环甘油三酯的关键酶，并且与某些病理状态如肥胖症有关。为了研究动物的内源性脂肪偏好是否与脂肪组织LPL的差异相关，我们测量了高脂偏好和低脂偏好大鼠附睾脂肪中的酶活性。利用24小时自由采食模式，对四组远交成年雄性Sprague-Dawley大鼠分别进行常量营养素偏好筛选。识别出表现出高脂或低脂偏好的动物，并将它们放回标准饲料喂养。在喂食或禁食条件下测量附睾脂肪中的LPL活性。在标准饲料喂养状态（p = 0.014）和禁食（p = 0.0007）状态下，高脂偏好动物的附睾脂肪LPL活性相对于低脂偏好动物均显著降低。还测量了同一批动物心室和棕色脂肪组织中的LPL活性。仅在禁食24小时后，高脂偏好组的心室和棕色脂肪组织中的活性与低脂偏好组相比显著降低（心室p = 0.0012，棕色脂肪p = 0.0085，高脂与低脂偏好）。数据表明，脂肪偏好存在固有差异的动物之间组织LPL活性存在差异。未来对两组脂肪偏好动物的比较研究可能会为LPL的调节提供重要线索。
