Alanko J, Riutta A, Vapaatalo H
Department of Biomedical Sciences, University of Tampere, Finland.
Prostaglandins Leukot Essent Fatty Acids. 1995 May;52(5):299-301. doi: 10.1016/0952-3278(95)90030-6.
We have previously demonstrated that the phenolic compounds catechol, hydroquinone, and phenol increase the prostaglandin (PG) E2/leukotriene (LT) B4 ratio in human polymorphonuclear leukocytes (PMNs), while resorcinol has the opposite effect. However, in human whole blood phenols have a different effect on the thromboxane (TX) B2/LT ratio than in PMNs on the PGE2/LTB4 ratio. To establish whether the discrepancy between the results of our previous studies is due to different indicators of prostaglandin H synthase activity in PMNs (PGE2) and in whole blood (TXB2), we measured the effect of phenols on PGE2 synthesis in whole blood. The phenols only inhibited PGE2 synthesis (IC50 values for resorcinol, catechol, hydroquinone, and phenol of 10 microM, 10 microM, 60 microM and 700 microM, respectively). No significant stimulatory activity was seen as earlier in PMNs. Thus, the effect of phenols on PGE2 synthesis in whole blood is different from that in PMNs, although their order of potency to inhibit PGE2 synthesis is the same.
我们之前已经证明，酚类化合物邻苯二酚、对苯二酚和苯酚可提高人多形核白细胞（PMN）中前列腺素（PG）E2/白三烯（LT）B4的比值，而间苯二酚则具有相反的作用。然而，在人全血中，酚类对血栓素（TX）B2/LT比值的影响与在PMN中对PGE2/LTB4比值的影响不同。为了确定我们之前研究结果之间的差异是否是由于PMN中（PGE2）和全血中（TXB2）前列腺素H合酶活性的指标不同，我们测定了酚类对全血中PGE2合成的影响。酚类仅抑制PGE2的合成（间苯二酚、邻苯二酚、对苯二酚和苯酚的IC50值分别为10 microM、10 microM、60 microM和700 microM）。未观察到如之前在PMN中所见的显著刺激活性。因此，酚类对全血中PGE2合成的影响与对PMN中的影响不同，尽管它们抑制PGE2合成的效力顺序相同。
