Saareks V, Mucha I, Sievi E, Vapaatalo H, Riutta A
Department of Pharmacological Sciences, University of Tampere, Finland.
Eur J Pharmacol. 1998 Jul 17;353(1):87-92. doi: 10.1016/s0014-2999(98)00384-7.
The effects of (-)-nicotine (0.0005-500 microM), (+)-nicotine (0.0005-50 microM) and (-)-cotinine (0.0005-500 microM) on arachidonic acid metabolism were investigated in Ca2+ ionophore A23187 (calcimycin)-stimulated human whole blood in vitro. (-)-Nicotine and (-)-cotinine stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis, as has been observed previously in A23187-stimulated polymorphonuclear leukocytes and platelet-rich plasma [Saareks, V., Riutta, A., Mucha, I., Alanko, J., Vapaatalo, H., 1993. Nicotine and cotinine modulate eicosanoid production in human leukocytes and platelet rich plasma. Eur. J. Pharmacol., 248, 345-349.]. (+)-Nicotine also stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis. High concentrations of (-)-nicotine and (-)-cotinine and even nanomolar concentrations of (+)-nicotine inhibited leukotriene E4 synthesis. These results indicate that (-)-nicotine and (-)-cotinine stimulate cyclooxygenase but inhibit thromboxane synthase and 5-lipoxygenase in whole blood in vitro. (+)-Nicotine is capable of affecting in the same direction as well.
在体外，使用钙离子载体A23187（钙霉素）刺激人全血，研究了（-）-尼古丁（0.0005 - 500微摩尔）、（+）-尼古丁（0.0005 - 50微摩尔）和（-）-可替宁（0.0005 - 500微摩尔）对花生四烯酸代谢的影响。如先前在A23187刺激的多形核白细胞和富含血小板的血浆中所观察到的那样，（-）-尼古丁和（-）-可替宁刺激前列腺素E2的生成，但抑制血栓素B2的合成[萨雷克，V.，留塔，A.，穆哈，I.，阿兰科，J.，瓦帕塔洛，H.，1993年。尼古丁和可替宁调节人白细胞和富含血小板血浆中类二十烷酸的产生。《欧洲药理学杂志》，248，345 - 349。]。（+）-尼古丁也刺激前列腺素E2的生成，但抑制血栓素B2的合成。高浓度的（-）-尼古丁和（-）-可替宁，甚至纳摩尔浓度的（+）-尼古丁都抑制白三烯E4的合成。这些结果表明，在体外人全血中，（-）-尼古丁和（-）-可替宁刺激环氧化酶，但抑制血栓素合酶和5-脂氧合酶。（+）-尼古丁也能够在相同方向上产生影响。
