Cassinotti P, Bas S, Siegl G, Vischer T L
Institute for Clinical Microbiology and Immunology, St Gallen, Switzerland.
Ann Rheum Dis. 1995 Jun;54(6):498-500. doi: 10.1136/ard.54.6.498.
To gain information concerning the association between parvovirus B19 infection and arthritis.
Blood or synovial fluid, or both, from a total of 77 adult patients with various arthropathies (rheumatoid arthritis 13; mechanical arthropathies 11; crystal induced arthritis 13; idiopathic mono/oligoarthritis 25; suspicion of viral arthritis 15) were tested for the presence of the viral genome and anti-B19 antibodies. B19 DNA in blood and synovial fluid was investigated by nested polymerase chain reaction, and anti-B19 IgM and IgG antibodies were detected in blood by enzyme immunoassay.
A recent parvovirus infection was documented by the presence of anti-B19 IgM antibodies in the blood of 13 patients. B19 DNA, together with anti-B19 IgM and IgG antibodies, were detected in the blood of seven patients who had an acute transient arthritis, putatively of viral origin. Viral DNA was detected in a synovial fluid sample and in the blood of one patient with monoarthritis who had an anti-B19 IgG response only.
The prevalence of anti-B19 IgG antibody in these patients with various forms of arthritis (63%) was within the same range as that in the general population (blood donors). However, for the patients with clinical suspicion of viral arthritis, the increased seroprevalence of anti-B19 IgM and the presence of the B19 genome point to an association between human parvovirus infections and acute forms of arthritis.
获取有关细小病毒B19感染与关节炎之间关联的信息。
对总共77例患有各种关节病的成年患者（类风湿性关节炎13例；机械性关节病11例；晶体诱导性关节炎13例；特发性单/寡关节炎25例；疑似病毒性关节炎15例）的血液或滑液，或两者进行检测，以确定病毒基因组和抗B19抗体的存在情况。通过巢式聚合酶链反应研究血液和滑液中的B19 DNA，并通过酶免疫测定法检测血液中的抗B19 IgM和IgG抗体。
13例患者血液中存在抗B19 IgM抗体，证明近期有细小病毒感染。在7例患有急性短暂性关节炎（推测为病毒源性）的患者血液中检测到B19 DNA以及抗B19 IgM和IgG抗体。在一名仅产生抗B19 IgG反应的单关节炎患者的滑液样本和血液中检测到病毒DNA。
这些患有各种形式关节炎的患者中抗B19 IgG抗体的患病率（63%）与普通人群（献血者）处于同一范围。然而，对于临床疑似病毒性关节炎的患者，抗B19 IgM血清阳性率的增加以及B19基因组的存在表明人类细小病毒感染与急性关节炎形式之间存在关联。
