Nonogaki T, Noda Y, Goto Y, Kishi J, Mori T
Department of Gynecology and Obstetrics, Kyoto University, Faculty of Medicine, Japan.
J Assist Reprod Genet. 1994 Oct;11(9):482-8. doi: 10.1007/BF02215713.
It has been shown that lipid peroxides derived from polyunsaturated fatty acids (PUFAs) inhibit the proliferation of various cells. In the meantime, it has been suggested that oxidative stress is closely related to the developmental blockage of mammalian embryos cultured in vitro. In this study, we investigated the effects by various fatty acids on mouse embryo development in vitro, and the reversal of these effects by various antioxidants such as superoxide dismutase, ascorbic acid, alpha-tocopherol, uric acid, and ethylenediaminetetraacetic acid.
Pronuclear and two-cell stage mouse (ICR) embryos were cultured in Biggers-Whitten-Whittingham medium with 0.3% bovine serum albumin alone or complexed with one of the following fatty acids: palmitic, stearic, oleic, linoleic, linolenic, or arachidonic acid. We also measured the fluorescence emission of embryos in media containing various fatty acids in order to investigate the involvement of H2O2 or lipid peroxidation in embryo development.
Palmitic acid and PUFAs including linoleic acid inhibited the embryo development. The inhibitory effect of PUFAs was attenuated by adding antioxidants into the media, while the inhibitory effect of palmitic acid was not. Both pronuclear and two-cell stage embryos with PUFAs showed markedly more intensive emissions than those under other conditions.
These results suggest that lipid radicals can easily be generated in early stage embryos and that blastomeres are among the cells vulnerable to the damage by lipid peroxidation.
研究表明，多不饱和脂肪酸（PUFA）衍生的脂质过氧化物可抑制多种细胞的增殖。同时，有研究表明氧化应激与体外培养的哺乳动物胚胎发育阻滞密切相关。在本研究中，我们调查了不同脂肪酸对小鼠胚胎体外发育的影响，以及超氧化物歧化酶、抗坏血酸、α-生育酚、尿酸和乙二胺四乙酸等多种抗氧化剂对这些影响的逆转作用。
将原核期和二细胞期小鼠（ICR）胚胎培养在含有0.3%牛血清白蛋白的Biggers-Whitten-Whittingham培养基中，该培养基单独使用或与下列脂肪酸之一复合：棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸或花生四烯酸。我们还测量了含有不同脂肪酸的培养基中胚胎的荧光发射，以研究H2O2或脂质过氧化在胚胎发育中的作用。
棕榈酸和包括亚油酸在内的多不饱和脂肪酸抑制胚胎发育。向培养基中添加抗氧化剂可减弱多不饱和脂肪酸的抑制作用，而棕榈酸的抑制作用则不受影响。与其他条件下的胚胎相比，含有多不饱和脂肪酸的原核期和二细胞期胚胎均表现出明显更强的荧光发射。
这些结果表明，脂质自由基很容易在早期胚胎中产生，卵裂球是易受脂质过氧化损伤的细胞之一。
