Nagao K, Gomyo Y, Ito H, Yamamoto K, Yoshida H
Department of Pathology, Faculty of Medicine, Tottori University, Japan.
Virchows Arch. 1995;426(5):519-22. doi: 10.1007/BF00193176.
A 57-year-old female patient with synovial sarcoma in her right foot had a chromosome abnormality defined as translocation (X;18). The tumour was located in the subcutis, and histological investigation showed monophasic proliferation of oval to spindle-shaped cells with a fascicular arrangement lacking an epithelial component. Immunostaining disclosed no cytokeratin or epithelial membrane antigen in tumour cells. Karyotypic analysis revealed translocation (X;18) in addition to other nonspecific aberrations. Fluorescence in situ hybridization was carried out on paraffin-embedded tissue, using DNA probes for the centromeres of chromosomes X and 18 with whole chromosome painting probes for X and 18. The free nuclei showed two signals at a rate of 83-85% with the X and 18 centromeric probes, in contrast to three signals at a rate of 68-70% with the X and 18 painting probes.
一名57岁的女性患者，右脚患有滑膜肉瘤，其染色体异常表现为易位（X；18）。肿瘤位于皮下，组织学检查显示为椭圆形至梭形细胞的单相增殖，呈束状排列，缺乏上皮成分。免疫染色显示肿瘤细胞中无细胞角蛋白或上皮膜抗原。核型分析显示除其他非特异性畸变外，还存在易位（X；18）。对石蜡包埋组织进行荧光原位杂交，使用针对X和18号染色体着丝粒的DNA探针以及针对X和18号染色体的全染色体涂染探针。游离核在用X和18号着丝粒探针检测时，83 - 85%的细胞核显示两个信号，而在用X和18号涂染探针检测时，68 - 70%的细胞核显示三个信号。
