Hirakawa N, Naka T, Yamamoto I, Fukuda T, Tsuneyoshi M
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hum Pathol. 1996 Oct;27(10):1060-5. doi: 10.1016/s0046-8177(96)90284-1.
The expression of bcl-2 protein was analyzed in 19 synovial sarcomas and 29 additional soft tissue spindle cell sarcomas as controls by the immunohistologic staining of paraffin-embedded specimens; 15 of 19 (79%) synovial sarcoma cases were positive, but all other spindle cell sarcomas were negative including 20 leiomyosarcomas, 4 malignant peripheral nerve sheath tumors, and 4 fibrosarcomas. In 4 cases of bcl-2-positive synovial sarcoma (3 biphasic and 1 focally glandular type), bcl-2 protein staining was much stronger in the spindle cells than in the epithelial cells. A fluorescence in situ hybridization (FISH) analysis with centromeric and whole chromosome painting probes for chromosome 18 and X was performed on 7 synovial sarcomas. The six cases of bcl-2-positive synovial sarcoma consisted of five cases with the t(X; 18) and one case with tetrasomy of chromosome 18 and X. It has been speculated that bcl-2 protein expression is caused by the 14; 18 translocation and other abnormalities of chromosome 18. This study thus showed the feasibility of such a correlation between bcl-2 protein expression and the characteristic cytogenetic abnormality in the synovial sarcoma-X; 18 translocation. In addition, bcl-2 protein also appears to be a characteristic marker of synovial sarcoma and is thus considered to be potentially useful in distinguishing synovial sarcoma from other spindle cell sarcomas.
通过对石蜡包埋标本进行免疫组织化学染色，分析了19例滑膜肉瘤和另外29例软组织梭形细胞肉瘤（作为对照）中bcl-2蛋白的表达情况；19例滑膜肉瘤中有15例（79%）呈阳性，但所有其他梭形细胞肉瘤均为阴性，包括20例平滑肌肉瘤、4例恶性外周神经鞘瘤和4例纤维肉瘤。在4例bcl-2阳性的滑膜肉瘤（3例双相型和1例局灶性腺型）中，bcl-2蛋白在梭形细胞中的染色比上皮细胞强得多。对7例滑膜肉瘤进行了用18号染色体和X染色体着丝粒及全染色体涂染探针的荧光原位杂交（FISH）分析。6例bcl-2阳性的滑膜肉瘤中，5例存在t(X;18)，1例存在18号染色体和X染色体四体。据推测，bcl-2蛋白表达是由14;18易位和18号染色体的其他异常引起的。因此，本研究表明了滑膜肉瘤中bcl-2蛋白表达与特征性细胞遗传学异常-X;18易位之间存在这种相关性的可行性。此外，bcl-2蛋白似乎也是滑膜肉瘤的一个特征性标志物，因此被认为在鉴别滑膜肉瘤与其他梭形细胞肉瘤方面可能有用。
