Villani L, Guarnieri T
Department of Biology, University of Bologna, Italy.
Brain Res. 1995 May 15;679(2):261-6. doi: 10.1016/0006-8993(95)00240-q.
The distribution of NADPH-diaphorase positive neurons was studied by using the enzyme histochemical method. Numerous neurons were labeled in different brain areas of the goldfish and their distribution showed some differences in comparison with other studied teleosts, indicating a species-specific pattern of NADPH-diaphorase distribution as observed in mammals. The localization of NADPH-diaphorase in the thalamic nuclei, in the paraventricular organ, in the inferior hypothalamic lobe, in the periventricular neurons of the optic tectum, in the nucleus isthmi and in the mesencephalic reticular formation was comparable to the one observed in other teleosts. In addition in the goldfish the telencephalic neurons of the pars centralis and lateralis of the area dorsalis, the habenular neurons, the bipolar neurons of the central grey layer of the optic tectum and the motor neurons of the hypertrophied vagal lobe were labeled. The localization of NADPH-diaphorase positive neurons, compared to the distribution of cholinergic neurons described in fish, indicated that the production of nitric oxide was prevailing in the brain areas where cholinergic circuits are active.
采用酶组织化学方法研究了NADPH - 黄递酶阳性神经元的分布。在金鱼的不同脑区标记了大量神经元，与其他已研究的硬骨鱼相比，它们的分布存在一些差异，这表明在哺乳动物中观察到的NADPH - 黄递酶分布具有物种特异性模式。NADPH - 黄递酶在丘脑核、室旁器官、下丘脑下部叶、视顶盖室周神经元、峡核和中脑网状结构中的定位与在其他硬骨鱼中观察到的相似。此外，在金鱼中，背侧区中央部和外侧部的端脑神经元、缰核神经元、视顶盖中央灰质层的双极神经元以及肥大迷走叶的运动神经元也被标记。与鱼类中描述的胆碱能神经元分布相比，NADPH - 黄递酶阳性神经元的定位表明，在胆碱能回路活跃的脑区中一氧化氮的产生占主导地位。
