Villani L, Guarnieri T
Department of Biology, University of Bologna, Italy.
Brain Res. 1995 Dec 24;705(1-2):332-6. doi: 10.1016/0006-8993(95)01251-6.
The ultrastructural localization of NADPH-diaphorase was studied in the goldfish brain by means of the tetrazolium salt technique. The reaction product was found to be bound to the endoplasmic membranes of neurons in different brain areas. In the synaptic structures both pre and post-synaptic profiles showed the reaction product. Furthermore non-neuronal structures were intensely labeled. Endothelial cells revealed the membranous localization of NADPH-diaphorase and the glial cells of the hypothalamic nuclei and of the paraventricular organ were labeled. In some cases the reaction product was seen also in oligodendrocytes and in microglial cells.
采用四氮唑盐技术研究了金鱼脑中NADPH-黄递酶的超微结构定位。发现反应产物与不同脑区神经元的内质网膜结合。在突触结构中，突触前和突触后成分均显示有反应产物。此外，非神经元结构也被强烈标记。内皮细胞显示出NADPH-黄递酶的膜定位，下丘脑核和室旁器官的神经胶质细胞也被标记。在某些情况下，少突胶质细胞和小胶质细胞中也可见反应产物。
