一种功能性真菌聚酮合酶在天蓝色链霉菌A3(2)中的表达。
Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).
作者信息
Bedford D J, Schweizer E, Hopwood D A, Khosla C
机构信息
Department of Chemical Engineering, Stanford University, California 94305-5025, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4544-8. doi: 10.1128/jb.177.15.4544-4548.1995.
Abstract
The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.
摘要
来自展青霉的多功能6-甲基水杨酸合酶基因经改造后可在天蓝色链霉菌中实现调控表达。通过核磁共振波谱法证实重组菌株能产生大量的6-甲基水杨酸。这些结果表明，通过在一个易于操作的模式细菌宿主中异源表达生物合成基因，利用真核聚酮化合物途径的分子多样性是可能的，在该宿主中，原核芳香族和模块化聚酮合酶基因已经表达并重组。
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