两种独立的表达途径导致兔出血症病毒衣壳蛋白的自我组装。
Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein.
作者信息
Sibilia M, Boniotti M B, Angoscini P, Capucci L, Rossi C
机构信息
Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia, Brescia, Italy.
出版信息
J Virol. 1995 Sep;69(9):5812-5. doi: 10.1128/JVI.69.9.5812-5815.1995.
Abstract
The rabbit hemorrhagic disease virus capsid protein was expressed in insect cells either as an individual protein species, from a mRNA analogous to the viral subgenomic RNA, or as part of a polyprotein that included the viral 3C-like protease and the RNA polymerase. Both pathways of expression led to the assembly of viruslike particles morphologically and antigenically similar to purified virus.
摘要
兔出血症病毒衣壳蛋白在昆虫细胞中表达，既可以作为一种单独的蛋白质，由类似于病毒亚基因组RNA的mRNA表达产生，也可以作为一种多蛋白的一部分进行表达，该多蛋白包含病毒3C样蛋白酶和RNA聚合酶。这两种表达途径均导致了形态和抗原性上与纯化病毒相似的病毒样颗粒的组装。
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