对编码丙型肝炎病毒核心蛋白的质粒DNA的免疫反应。
Immune responses to plasmid DNA encoding the hepatitis C virus core protein.
作者信息
Lagging L M, Meyer K, Hoft D, Houghton M, Belshe R B, Ray R
机构信息
Division of Infectious Diseases and Immunology, Saint Louis University Health Sciences Center, MO 63104, USA.
出版信息
J Virol. 1995 Sep;69(9):5859-63. doi: 10.1128/JVI.69.9.5859-5863.1995.
Abstract
Hepatitis C virus (HCV) is a major causative agent of parenterally transmitted non-A, non-B hepatitis. The genomic region encoding the virion-associated core protein is relatively conserved among HCV strains. To generate a DNA vaccine capable of expressing the HCV core protein, the genomic region encoding amino acid residues 1 to 191 of the HCV-1 strain was amplified and cloned into an eukaryotic expression vector. Intramuscular inoculation of recombinant plasmid DNA into BALB/c mice (H-2d) generated core-specific antibody responses, lymphoproliferative responses, and cytotoxic T-lymphocyte activity. Our results suggest that the HCV core polynucleotide warrants further investigation as a potential vaccine against HCV infection.
摘要
丙型肝炎病毒（HCV）是经肠道外传播的非甲非乙型肝炎的主要病原体。编码病毒体相关核心蛋白的基因组区域在HCV毒株中相对保守。为了构建一种能够表达HCV核心蛋白的DNA疫苗，将编码HCV-1毒株氨基酸残基1至191的基因组区域进行扩增，并克隆到真核表达载体中。将重组质粒DNA肌肉注射到BALB/c小鼠（H-2d）体内可产生核心特异性抗体反应、淋巴细胞增殖反应和细胞毒性T淋巴细胞活性。我们的结果表明，HCV核心多核苷酸作为一种潜在的抗HCV感染疫苗值得进一步研究。
相似文献
1
Immune responses to plasmid DNA encoding the hepatitis C virus core protein.对编码丙型肝炎病毒核心蛋白的质粒DNA的免疫反应。
J Virol. 1995 Sep;69(9):5859-63. doi: 10.1128/JVI.69.9.5859-5863.1995.
2
Expression and immune response to hepatitis C virus core DNA-based vaccine constructs.丙型肝炎病毒核心DNA疫苗构建体的表达及免疫反应
Hepatology. 1996 Jul;24(1):14-20. doi: 10.1002/hep.510240104.
3
Characterization of the humoral and cellular immune responses against hepatitis C virus core induced by DNA-based immunization.基于DNA免疫诱导的针对丙型肝炎病毒核心抗原的体液免疫和细胞免疫反应的特性分析
Vaccine. 1999 Aug 6;17(23-24):3160-70. doi: 10.1016/s0264-410x(99)00130-9.
4
DNA-based immunization with chimeric vectors for the induction of immune responses against the hepatitis C virus nucleocapsid.使用嵌合载体进行基于DNA的免疫接种以诱导针对丙型肝炎病毒核衣壳的免疫反应。
J Virol. 1995 Sep;69(9):5798-805. doi: 10.1128/JVI.69.9.5798-5805.1995.
5
Hepatitis B virus precore protein augments genetic immunizations of the truncated hepatitis C virus core in BALB/c mice.乙肝病毒前核心蛋白增强BALB/c小鼠中截短型丙肝病毒核心蛋白的基因免疫效果。
Hepatology. 2008 Jan;47(1):25-34. doi: 10.1002/hep.21992.
6
7
High efficiency prokaryotic expression and purification of a portion of the hepatitis C core protein and analysis of the immune response to recombinant protein in BALB/c mice.丙型肝炎核心蛋白部分片段的高效原核表达、纯化及BALB/c小鼠对重组蛋白免疫反应的分析
Viral Immunol. 1995;8(2):109-19. doi: 10.1089/vim.1995.8.109.
8
[Immune responses to recombinant expression plasmid encoding hepatitis C virus core antigen].对编码丙型肝炎病毒核心抗原的重组表达质粒的免疫反应
Zhonghua Nei Ke Za Zhi. 1999 Jul;38(7):462-5.
9
Additives and protein-DNA combinations modulate the humoral immune response elicited by a hepatitis C virus core-encoding plasmid in mice.
Mem Inst Oswaldo Cruz. 2002 Jan;97(1):95-9. doi: 10.1590/s0074-02762002000100016.
10
Effective induction of type 1 cytotoxic T cell responses in mice with DNA vaccine encoding two hepatitis C virus cytotoxic T lymphocyte epitopes.用编码两个丙型肝炎病毒细胞毒性T淋巴细胞表位的DNA疫苗在小鼠中有效诱导1型细胞毒性T细胞反应。
Viral Immunol. 2006 Winter;19(4):702-11. doi: 10.1089/vim.2006.19.702.
引用本文的文献
1
The Immunogenicity in Mice of HCV Core Delivered as DNA Is Modulated by Its Capacity to Induce Oxidative Stress and Oxidative Stress Response.HCV 核心蛋白作为 DNA 免疫原在小鼠体内的免疫原性可通过诱导氧化应激及其应答的能力来调节。
Cells. 2019 Feb 28;8(3):208. doi: 10.3390/cells8030208.
2
Design and expression of fusion protein consists of HBsAg and Polyepitope of HCV as an HCV potential vaccine.由乙肝表面抗原（HBsAg）和丙型肝炎病毒（HCV）多表位组成的融合蛋白作为一种潜在的丙型肝炎病毒疫苗的设计与表达。
Adv Biomed Res. 2015 Oct 29;4:243. doi: 10.4103/2277-9175.168610. eCollection 2015.
3
Inhibition of the HCV core protein on the immune response to HBV surface antigen and on HBV gene expression and replication in vivo.HCV 核心蛋白对 HBV 表面抗原免疫反应及 HBV 基因表达和复制的体内抑制作用。
PLoS One. 2012;7(9):e45146. doi: 10.1371/journal.pone.0045146. Epub 2012 Sep 14.
4
The wild-type hepatitis C virus core inhibits initiation of antigen-specific T- and B-cell immune responses in BALB/c mice.野生型丙型肝炎病毒核心蛋白抑制BALB/c小鼠抗原特异性T细胞和B细胞免疫反应的启动。
Clin Vaccine Immunol. 2010 Jul;17(7):1139-47. doi: 10.1128/CVI.00490-09. Epub 2010 Jun 2.
5
Construction and immunological evaluation of multivalent hepatitis B virus (HBV) core virus-like particles carrying HBV and HCV epitopes.携带乙肝病毒（HBV）和丙肝病毒（HCV）表位的多价乙肝病毒核心病毒样颗粒的构建及免疫学评估
Clin Vaccine Immunol. 2010 Jun;17(6):1027-33. doi: 10.1128/CVI.00468-09. Epub 2010 Apr 21.
6
Identification and structural definition of H5-specific CTL epitopes restricted by HLA-A*0201 derived from the H5N1 subtype of influenza A viruses.鉴定和结构定义的 H5 特异性 CTL 表位受限制的 HLA-A*0201 从 H5N1 亚型的流感病毒。
J Gen Virol. 2010 Apr;91(Pt 4):919-30. doi: 10.1099/vir.0.016766-0. Epub 2009 Dec 2.
7
Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen.丙型肝炎病毒核心基因表达增强在DNA免疫中未增强核心特异性免疫反应：异源DNA初免、蛋白加强免疫方案的优势
Genet Vaccines Ther. 2009 Jun 8;7:7. doi: 10.1186/1479-0556-7-7.
8
Characterization of cytotoxic T-lymphocyte epitopes and immune responses to SARS coronavirus spike DNA vaccine expressing the RGD-integrin-binding motif.表达RGD-整合素结合基序的SARS冠状病毒刺突DNA疫苗的细胞毒性T淋巴细胞表位特征及免疫反应
J Med Virol. 2009 Jul;81(7):1131-9. doi: 10.1002/jmv.21571.
9
Vaccines to prevent chronic hepatitis C virus infection: current experimental and preclinical developments.预防慢性丙型肝炎病毒感染的疫苗：当前的实验和临床前进展
J Gastroenterol. 2007 Jun;42(6):424-32. doi: 10.1007/s00535-007-2057-5. Epub 2007 Jun 29.
10
Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.在BALB/c小鼠中，用编码严重急性呼吸综合征冠状病毒不同基因片段的DNA疫苗引发免疫反应。
Biochem Biophys Res Commun. 2005 Feb 4;327(1):130-5. doi: 10.1016/j.bbrc.2004.11.147.
本文引用的文献
1
Vaccination to prevent persistent viral infection.通过接种疫苗预防持续性病毒感染。
J Virol. 1993 Jul;67(7):4372-8. doi: 10.1128/JVI.67.7.4372-4378.1993.
2
Gene inoculation generates immune responses against human immunodeficiency virus type 1.基因接种可引发针对1型人类免疫缺陷病毒的免疫反应。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4156-60. doi: 10.1073/pnas.90.9.4156.
3
Heterologous protection against influenza by injection of DNA encoding a viral protein.通过注射编码病毒蛋白的DNA实现对流感的异源保护。
Science. 1993 Mar 19;259(5102):1745-9. doi: 10.1126/science.8456302.
4
Naked DNA points way to vaccines.
Science. 1993 Mar 19;259(5102):1691-2. doi: 10.1126/science.8456293.
5
Bovine herpesvirus 1: immune responses in mice and cattle injected with plasmid DNA.牛疱疹病毒1型：注射质粒DNA的小鼠和牛的免疫反应
J Virol. 1993 Sep;67(9):5664-7. doi: 10.1128/JVI.67.9.5664-5667.1993.
6
DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations.DNA疫苗：通过肠胃外、粘膜及基因枪接种实现的保护性免疫
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11478-82. doi: 10.1073/pnas.90.24.11478.
7
Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA.通过用表达血凝素的质粒DNA免疫来抵御致死性流感病毒攻击。
Vaccine. 1993;11(9):957-60. doi: 10.1016/0264-410x(93)90385-b.
8
Peptide immunogen mimicry of putative E1 glycoprotein-specific epitopes in hepatitis C virus.丙型肝炎病毒中假定的E1糖蛋白特异性表位的肽免疫原模拟
J Virol. 1994 Jul;68(7):4420-6. doi: 10.1128/JVI.68.7.4420-4426.1994.
9
Hepatitis viruses: changing patterns of human disease.肝炎病毒：人类疾病模式的变化
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2401-6. doi: 10.1073/pnas.91.7.2401.
10
Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus.用携带狂犬病病毒糖蛋白基因的质粒载体进行疫苗接种可诱导针对狂犬病病毒的保护性免疫。
Virology. 1994 Feb 15;199(1):132-40. doi: 10.1006/viro.1994.1105.
Zotero 插件