Girard J P, Fernandes B
Z Erkr Atmungsorgane. 1977 Aug;149(2):265-70.
Various technical procedure were applied to the purification of T and B lymphocytes subpopulation in sarcoidosis. Patients were selected according to the presence of a characterized deficit in cell-mediated immune functions. These purified lymphocytes populations were further analysed for their ability to release lymphokines in the presence of Con-A-sepharose or specific antigens. Among these factors, MIF, LIF and mitogenic factor (MF) were looked for. For the determination of MIF and LIF, a newly elaborated technique was deviced which allows a semi-quantitative approach of the lymphokine production. The following results were obtained: A decrease in the absolute number of T lymphocytes was observed among all our patients, and a increase in the number of Ig producing cells. A rather large percent (15 to 40%) of null cells which were carrying characteristic features of quiescent, non activable monocytes. The ability of T lymphocytes to release lymphokines was impaired in the presence of these monocytes but could be restored to normal levels when cells were removed. The results suggest that T cell function might be impaired, in sarcoidosis, by a non identified monocytes abnormal behaviour.
采用了各种技术程序来纯化结节病中的T和B淋巴细胞亚群。根据细胞介导免疫功能存在特征性缺陷来选择患者。对这些纯化的淋巴细胞群体进一步分析其在存在伴刀豆球蛋白A-琼脂糖或特异性抗原的情况下释放淋巴因子的能力。在这些因子中，寻找移动抑制因子（MIF）、淋巴细胞抑制因子（LIF）和促有丝分裂因子（MF）。为了测定MIF和LIF，设计了一种新的技术，该技术允许对淋巴因子产生进行半定量分析。获得了以下结果：在我们所有患者中观察到T淋巴细胞绝对数量减少，以及产生免疫球蛋白细胞数量增加。相当大比例（15%至40%）的裸细胞具有静止、不可激活单核细胞的特征。在这些单核细胞存在的情况下，T淋巴细胞释放淋巴因子的能力受损，但当去除细胞时可恢复到正常水平。结果表明，在结节病中，T细胞功能可能因未确定的单核细胞异常行为而受损。
