Gormus B J, Xu K, Cho S N, Baskin G B, Bohm R P, Martin L N, Blanchard J L, Mack P A, Ratterree M S, Meyers W M
Department of Microbiology, Tulane Regional Primate Research Center, Covington, LA, USA.
Lepr Rev. 1995 Jun;66(2):105-25. doi: 10.5935/0305-7518.19950013.
In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin Mycobacterium leprae (ML) between 4.5 x 10(8) and 1 x 10(9) by either combined IV/IC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of 1 x 10(9) ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority of leprosy-susceptible SMM in contrast to negative levels in more resistant animals. Anti-LAM IgG OD values exceeded the positive cut-off point after inoculation in 5 of 11 SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen.
在本研究中，11只严重麻风反应小鼠（SMM）被分组，并通过静脉/腹腔联合途径或单独的静脉或腹腔途径，接种4.5×10⁸至1×10⁹之间不同剂量的源自SMM的麻风分枝杆菌（ML）。联合途径在引发进行性播散性瘤型麻风（LL）方面最有效。总共，通过联合途径接种的7只SMM中有6只在某个时候患上了需要治疗的麻风病。通过单一途径接种的4只中只有1只患上了需要化疗的持续性麻风病。通过单一途径接种的其他3只动物要么没有发病，要么初始疾病自发消退。超过1×10⁹ ML的剂量比小剂量更有效。观察到IgG抗酚糖脂-I ELISA OD值的发展与对麻风病的抵抗力之间以及IgM抗酚糖脂-I与麻风病进展或易感性之间存在关联。通过斑点ELISA测定的血清酚糖脂-I抗原水平在纵向上与疾病严重程度平行。与更具抵抗力的动物中的阴性水平相反，在大多数易感麻风病的SMM中，在接种ML之前观察到抗脂阿拉伯甘露聚糖（LAM）IgG的高阳性OD值。11只SMM中有5只在接种后抗LAM IgG OD值超过阳性临界点；这5只中的3只同时检测到血清酚糖脂-I抗原水平。
