Siems W G, Grune T, Esterbauer H
Herzog-Julius Hospital for Rheumatology and Orthopedics, Bad Harzburg, Germany.
Life Sci. 1995;57(8):785-9. doi: 10.1016/0024-3205(95)02006-5.
The metabolism and the washout of 4-hydroxynonenal (HNE), a major lipid peroxidation product, was determined in jejunal intestine of rats under normoxic, ischemic and postischemic conditions. The HNE utilization was by several orders higher than the HNE washout. Under ischemic conditions a drastic decrease of HNE utilization was found, compared with normoxic and postischemic utilization rates. The real flux rate of intracellular HNE formation during ischemia and reperfusion of small intestine was for the first time estimated on the basis of tissue HNE concentration, HNE utilization rate at defined HNE tissue concentration and HNE washout by perfusate circulation. Such estimation led to intestinal HNE formation rates of about 20 nmoles/g/min at normoxia, 20 to 40 nmoles/g/min at ischemia, 100 nmoles/g/min at 10 min of reperfusion and 20 nmoles/g/min at 60 min of reperfusion. Therefore, one can conclude that the tissue concentration of HNE only partly represents the real HNE formation rate and that the formation rate is of particular pathophysiological importance.
在常氧、缺血和缺血后条件下，测定了大鼠空肠中主要脂质过氧化产物4-羟基壬烯醛（HNE）的代谢及清除情况。HNE的利用比其清除高几个数量级。与常氧和缺血后利用率相比，在缺血条件下发现HNE利用急剧下降。首次基于组织HNE浓度、特定HNE组织浓度下的HNE利用率以及灌注液循环对HNE的清除，估算了小肠缺血和再灌注期间细胞内HNE形成的实际通量率。这种估算得出常氧时肠道HNE形成率约为20纳摩尔/克/分钟，缺血时为20至40纳摩尔/克/分钟，再灌注10分钟时为100纳摩尔/克/分钟，再灌注60分钟时为20纳摩尔/克/分钟。因此，可以得出结论，HNE的组织浓度仅部分代表实际HNE形成率，且该形成率具有特殊的病理生理重要性。
