Salagovic J, Kalina I, Dubayová K
Institute of Medical Biology, School of Medicine, P. J. Safárik University, Kosice, Slovakia.
Neoplasma. 1995;42(3):115-8.
An epidemiological study was performed on 42 workers from coke works with the aim to evaluate the usefulness of monitoring single strand DNA breaks (SSBs) in human lymphocytes to assess exposure to polycyclic aromatic hydrocarbons (PAHs) as well as the usefulness of SSBs induction as an indicator of biological effects of PAHs. SSBs can be readily quantitated by a simple fluorometric assay of DNA unwinding. Compared with the control group, statistically significant increase in SSBs was observed in coke oven workers occupationally exposed to PAHs. These findings are in agreement with previous results obtained by a different method of measuring DNA damage in subjects exposed to high PAH levels. The findings confirmed that SSBs determination in human lymphocytes reflected the exposure to PAHs and the FADU method appears to be useful in the revelation of effects of occupational exposure to industrial air pollutants such as PAHs.
对焦化厂的42名工人进行了一项流行病学研究，目的是评估监测人类淋巴细胞中单链DNA断裂（SSB）以评估多环芳烃（PAH）暴露的有用性，以及SSB诱导作为PAH生物效应指标的有用性。SSB可通过简单的DNA解旋荧光测定法轻松定量。与对照组相比，职业暴露于PAH的炼焦炉工人中观察到SSB有统计学意义的增加。这些发现与之前通过不同方法测量高PAH水平暴露受试者DNA损伤所获得的结果一致。研究结果证实，人类淋巴细胞中SSB的测定反映了PAH暴露情况，并且荧光DNA解旋法似乎有助于揭示职业暴露于PAH等工业空气污染物的影响。
