Kutinová L, Ludvíková V, Simonová V, Otavová M, Krystofová J, Hainz P, Press M, Kunke D, Vonka V
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Vaccine. 1995 Apr;13(5):487-93. doi: 10.1016/0264-410x(94)00019-j.
Three vaccinia virus strains (Praha, DD--a DRYVAX Wyeth vaccine-derived virus-and LIVP) were examined for growth in various cell cultures and for virulence and immunogenicity in mice. The viruses did not differ by their growth rates in monkey kidney cells (CV-1), human diploid cells (LEP), rat TK cells (RAT 2) or primary dog kidney cells. The immunogenicity of Praha and DD viruses was similar, the virus LIVP was somewhat more immunogenic. In terms of virulence in 3-day-old mice, the DD virus was the most attenuated. Single-plaque progenies were derived from the original smallpox vaccines VARIE Sevac (strain Praha) and DRYVAX Wyeth and tested for the above markers and DNA restriction patterns. The results obtained demonstrated biological and molecular heterogeneity of the original virus populations. Close linkage was observed between immunogenic activity and virulence in 3-day but not in 3-week mice. The results indicate that smallpox vaccine preparations may serve as an abundant source of virus mutants.
对三种痘苗病毒株（布拉格株、DD——一种源自惠氏DRYVAX疫苗的病毒——和LIVP株）进行了检测，观察它们在各种细胞培养物中的生长情况以及在小鼠体内的毒力和免疫原性。这些病毒在猴肾细胞（CV - 1）、人二倍体细胞（LEP）、大鼠TK细胞（RAT 2）或原代犬肾细胞中的生长速率没有差异。布拉格株和DD病毒的免疫原性相似，LIVP病毒的免疫原性略强。就对3日龄小鼠的毒力而言，DD病毒的减毒程度最高。单斑后代源自原始天花疫苗VARIE Sevac（布拉格株）和惠氏DRYVAX，并对上述标记物和DNA限制性图谱进行了检测。所得结果表明原始病毒群体存在生物学和分子异质性。在3日龄但不是3周龄小鼠中，观察到免疫原活性与毒力之间存在紧密联系。结果表明，天花疫苗制剂可能是病毒突变体的丰富来源。
