O'Connell T D, Simpson R U
Department of Pharmacology, University of Michigan, Ann Arbor 48109-0626, USA.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):59-65. doi: 10.1006/bbrc.1995.2098.
In this report we demonstrate that rats maintained on a calcium supplemented vitamin D3-deficient diet for nine weeks showed an increase in heart to body weight ratio. Morphometric analysis indicated that vitamin D3 deficiency produced cardiac myocytes that were smaller yet more numerous, indicating hyperplasia. Western blot analysis showed that vitamin D3 deficiency also resulted in increased c-myc protein levels in the hearts of vitamin D3-deficient rats. Furthermore, 1,25-dihydroxyvitamin D3 reduced c-myc protein levels in primary cultures of ventricular myocytes from neonatal rat hearts. Our data suggest a possible relationship between myocyte hyperplasia and increased c-myc levels in the vitamin D3-deficient rat heart.
在本报告中，我们证明，以补充钙但缺乏维生素D3的饮食喂养9周的大鼠，其心脏与体重之比有所增加。形态计量分析表明，维生素D3缺乏导致心肌细胞体积变小但数量增多，提示存在增生。蛋白质免疫印迹分析显示，维生素D3缺乏还导致维生素D3缺乏大鼠心脏中的c-myc蛋白水平升高。此外，1,25-二羟基维生素D3降低了新生大鼠心脏心室肌细胞原代培养物中的c-myc蛋白水平。我们的数据提示，在维生素D3缺乏的大鼠心脏中，心肌细胞增生与c-myc水平升高之间可能存在关联。
