Speulman E, Salamini F
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Plant Mol Biol. 1995 Aug;28(5):915-26. doi: 10.1007/BF00042075.
The barley mutant dbg 576 shows an extreme vegetative dwarf phenotype. This is reversed after the application of GA3 which also induces the mutant florets to become partly fertile. cDNA clones ES1A and ES2A were isolated by differential screening with subtracted probes from a DNA library prepared from mutant leaf blades after GA3 treatment. Both the ES1A and the ES2A mRNA level increases as early as 30 min after GA3 treatment and decreases later. Accumulation of ES1A and ES2A mRNAs is leaf blade-specific and both are ca. 750 nucleotides long. ES1A encodes a protein of approximately 6 kD which shows a significant homology with mammalian epidermal growth factors (EGFs). ES2A encodes a protein of 22 kDa with homology, in regions with potential amphiphilic helices, with the D7 family of late embryogenesis-abundant proteins (LEA).
大麦突变体dbg 576表现出极端的营养生长矮化表型。施用赤霉素（GA3）后这种表型会逆转，GA3还会诱导突变体小花部分可育。通过用来自GA3处理后突变体叶片制备的DNA文库的扣除探针进行差异筛选，分离出了cDNA克隆ES1A和ES2A。GA3处理后30分钟，ES1A和ES2A的mRNA水平就开始升高，随后下降。ES1A和ES2A mRNA的积累具有叶片特异性，二者长度均约为750个核苷酸。ES1A编码一种约6 kD的蛋白质，该蛋白质与哺乳动物表皮生长因子（EGF）具有显著同源性。ES2A编码一种22 kDa的蛋白质，在具有潜在两亲性螺旋的区域与晚期胚胎发育丰富蛋白（LEA）的D7家族具有同源性。
