Sado Y, Okigaki T
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
Cell Biol Int. 1996 Jan;20(1):7-14. doi: 10.1006/cbir.1996.0003.
A newly developed method using rat lymph nodes provided us with substantial amounts of high quality monoclonal antibodies. The method has characteristic features, a single injection of an emulsified antigen via the rat's hind footpads is sufficient for immunization. Enlarged medial iliac lymph nodes can be used for cell fusion to produce hybridomas 2 weeks and later after the antigen injection. The production frequency of target hybridomas is about 10 times higher than that of a conventional spleen method. Our current knowledge about the production of monoclonal antipeptide antibodies and monoclonal nephritogenic autoantibodies by the method is described.
一种新开发的利用大鼠淋巴结的方法为我们提供了大量高质量的单克隆抗体。该方法具有独特的特点，通过大鼠后足垫单次注射乳化抗原来进行免疫就足够了。注射抗原2周及之后，可使用肿大的髂内侧淋巴结进行细胞融合以产生杂交瘤。目标杂交瘤的产生频率比传统的脾脏方法高约10倍。本文描述了我们目前关于通过该方法生产单克隆抗肽抗体和单克隆致肾炎自身抗体的知识。
