Anderson D M, Ebersole J L, Novak M J
Department of Periodontics, University of Texas Health Science Center at San Antonio 78284, USA.
Infect Immun. 1995 Sep;63(9):3245-52. doi: 10.1128/iai.63.9.3245-3252.1995.
The nonhuman primate (NHP) serves as a useful model for examining the host-parasite interactions in Porphyromonas gingivalis-associated periodontal disease. This study determined the influence of NHP sera on (i) the direct killing of P. gingivalis, (ii) P. gingivalis-induced superoxide anion (O2-) release from human polymorphonuclear leukocytes (PMNs), and (iii) the ability of PMNs to bind and phagocytize P. gingivalis. Three types of NHP sera were utilized: (i) normal or baseline sera; (ii) sera obtained after ligature-induced periodontitis; and (iii) sera obtained following active immunization with formalinized P. gingivalis. All assays were performed with or without the addition of human complement. Significantly more (P < 0.01) direct killing of P. gingivalis occurred with immunized sera and complement than with any of the other treatments. The sera from ligature-induced periodontitis NHPs had significantly less (P < 0.03) killing capacity than the baseline sera, which contained natural antibody produced to P. gingivalis colonization. Sera from immunized NHPs were used to opsonize P. gingivalis and caused significantly greater (P < 0.01) levels of O2- release from PMNs. Finally, the sera from immunized NHPs significantly enhanced (P < 0.009) the uptake of P. gingivalis by PMNs, although binding of the bacteria to PMNs was similar among all three serum types. Active immunization of NHPs with P. gingivalis elicited a functional antibody that enhanced direct killing, positively influenced the activation of PMNs, and enhanced the ability of PMNs to phagocytize P. gingivalis. Moreover, antibody produced as a sequela of progressing periodontitis appeared to lack these functions. A wide variability in functional capacity of the sera from individual NHPs, which may contribute to an individual's susceptibility to P. gingivalis-induced disease, was noted. This variability suggested that results from functional tests of serum antibody may aid in predicting host susceptibility to disease and response to therapy.
非人灵长类动物（NHP）是研究牙龈卟啉单胞菌相关性牙周病中宿主 - 寄生虫相互作用的有用模型。本研究确定了NHP血清对以下方面的影响：（i）对牙龈卟啉单胞菌的直接杀伤作用；（ii）牙龈卟啉单胞菌诱导的人多形核白细胞（PMN）中超氧阴离子（O2-）的释放；（iii）PMN结合和吞噬牙龈卟啉单胞菌的能力。使用了三种类型的NHP血清：（i）正常或基线血清；（ii）结扎诱导牙周炎后获得的血清；（iii）用甲醛固定的牙龈卟啉单胞菌主动免疫后获得的血清。所有测定均在添加或不添加人补体的情况下进行。与任何其他处理相比，免疫血清和补体对牙龈卟啉单胞菌的直接杀伤作用显著更强（P < 0.01）。结扎诱导牙周炎的NHP血清的杀伤能力明显低于基线血清（P < 0.03），基线血清中含有针对牙龈卟啉单胞菌定植产生的天然抗体。免疫NHP的血清用于调理牙龈卟啉单胞菌，并导致PMN释放的O2-水平显著更高（P < 0.01）。最后，免疫NHP的血清显著增强了PMN对牙龈卟啉单胞菌的摄取（P < 0.009），尽管三种血清类型中细菌与PMN的结合相似。用牙龈卟啉单胞菌对NHP进行主动免疫可引发一种功能性抗体，该抗体增强直接杀伤作用，对PMN的激活产生积极影响，并增强PMN吞噬牙龈卟啉单胞菌的能力。此外，作为进展性牙周炎后遗症产生的抗体似乎缺乏这些功能。注意到个体NHP血清的功能能力存在广泛差异，这可能导致个体对牙龈卟啉单胞菌诱导疾病的易感性。这种差异表明，血清抗体功能测试的结果可能有助于预测宿主对疾病的易感性和对治疗的反应。
