Tanaka H, Manske P R, Pruitt D L, Larson B J
Division of Orthopedic Surgery, Washington University Medical Center, St. Louis, MO 63110, USA.
J Hand Surg Am. 1995 May;20(3):467-73. doi: 10.1016/S0363-5023(05)80109-1.
The effect of tension on tendon healing was evaluated in vitro using cyclic tension and no tension groups of chicken flexor digitorum profundus tendons and histologic and immunohistologic techniques. A Vitrodyne force-loading machine was used for application of cyclic tension on partially lacerated chicken flexor tendons in culture media. Laceration sites under cyclic tension after 14 days were covered by newly proliferated fibroblasts, aligned in the direction of tension. This new growth was much thicker than that seen in the no tension group at the same time interval. Procollagen synthetic activity began at 3 days of culture in both groups. At 21 days, newly formed fibroblasts in the cyclic tension group were stained positive more strongly at the surface layer than in the deeper layers. In the no tension group, the staining was primarily in the surface layer. Cyclic tension stimulated the intrinsic response of lacerated flexor tendons significantly more than no tension did by enhancing proliferation and migration of fibroblasts, as well as stimulating collagen synthesis.
使用鸡趾深屈肌腱的循环张力组和无张力组，以及组织学和免疫组织学技术，在体外评估张力对肌腱愈合的影响。使用维特罗德力加载机对培养基中部分撕裂的鸡屈肌腱施加循环张力。14天后，循环张力下的撕裂部位被新增殖的成纤维细胞覆盖，这些成纤维细胞沿张力方向排列。在相同时间间隔内，这种新生长比无张力组厚得多。两组在培养3天时开始有前胶原合成活性。在21天时，循环张力组新形成的成纤维细胞在表层的染色比深层更强。在无张力组中，染色主要在表层。循环张力通过增强成纤维细胞的增殖和迁移以及刺激胶原蛋白合成，比无张力更显著地刺激了撕裂屈肌腱的内在反应。
