Arany I, Nagamani K, Tyring S K
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA.
Anticancer Res. 1995 May-Jun;15(3):1003-6.
Interferons (IFNs) are successfully used in treatment of different human papillomavirus (HPV)-related diseases, such as condyloma acuminatum. Unresponsiveness can be seen in a number of patients which is related to a differential expression of early (E7) and late (L1) viral genes, according to our preliminary studies rather than to impaired IFN-signalling. The molecular basis for this differential expression might imply differential viral replication (copy numbers) in responder vs. nonresponder patients. PCR analysis revealed that the two groups did not differ significantly in HPV copy numbers before treatment. In contrast E7 and L1 levels significantly differed in responders versus nonresponders, regardless of copy numbers. Also, the IFN-mediated antiproliferative effect was mostly influenced by other factors rather than just the copy number. Our data imply that the unresponsiveness of certain patients to IFN treatment may relate to differential viral gene transcription rather than different copy numbers of infecting HIVs.
干扰素（IFNs）已成功用于治疗多种与人类乳头瘤病毒（HPV）相关的疾病，如尖锐湿疣。根据我们的初步研究，许多患者出现无反应性，这与病毒早期（E7）和晚期（L1）基因的差异表达有关，而非与干扰素信号传导受损有关。这种差异表达的分子基础可能意味着在有反应者与无反应者患者中病毒复制（拷贝数）存在差异。PCR分析显示，两组在治疗前HPV拷贝数上无显著差异。相比之下，无论拷贝数如何，有反应者与无反应者的E7和L1水平存在显著差异。此外，干扰素介导的抗增殖作用主要受其他因素影响，而非仅仅受拷贝数影响。我们的数据表明，某些患者对干扰素治疗无反应可能与病毒基因转录差异有关，而非与感染的HPV拷贝数不同有关。
