Arany I, Rady P, Tyring S K
Department of Microbiology, University of Texas Medical Branch, Galveston 77555-1019, USA.
Am J Med Sci. 1995 Jul;310(1):14-8. doi: 10.1097/00000441-199507000-00006.
Interferons exert antiviral, antiproliferative, and immunomodulatory effects on target cells. The effectiveness of interferon treatment can be followed by measuring parameters involved in interferon action. The clinical effectiveness of interferons has been proved in the human papillomavirus (HPV)-associated disease condyloma acuminatum. Because one of the most important goals in the treatment of this condition is the elimination of recurrences, it was asked whether clinically effective interferon therapy was associated with elimination of HPV DNA. The authors used a polymerase chain reaction-based method to detect the HPV from minute amounts of clinical biopsies. Human papillomavirus-transformed cell lines or cloned HPV genomes served as different copy number controls. The intensity ratio of L1 HPV and the human beta-globin (which served as an internal control) fragments was determined and used for estimation of HPV copy number in different biopsies. A direct effect of interferon treatment on HPV viral genome copies was observed in a group of responder patients, but not in the clinically resistant group. With these data, a correlation was found between virologic data and clinical findings. This method also can be used in other lesions to determine the HPV copy number and HPV type and may have value in determining the antiviral activities of other agents used in the treatment of HPV-related lesions.
干扰素对靶细胞发挥抗病毒、抗增殖和免疫调节作用。可通过测量与干扰素作用相关的参数来跟踪干扰素治疗的效果。干扰素的临床疗效已在人乳头瘤病毒（HPV）相关疾病尖锐湿疣中得到证实。由于治疗这种疾病的最重要目标之一是消除复发，因此有人提出临床有效的干扰素治疗是否与消除HPV DNA有关。作者使用基于聚合酶链反应的方法从微量临床活检样本中检测HPV。人乳头瘤病毒转化的细胞系或克隆的HPV基因组用作不同拷贝数的对照。测定L1 HPV与人类β-珠蛋白（用作内对照）片段的强度比，并用于估计不同活检样本中的HPV拷贝数。在一组有反应的患者中观察到干扰素治疗对HPV病毒基因组拷贝有直接影响，但在临床耐药组中未观察到。根据这些数据，发现病毒学数据与临床结果之间存在相关性。该方法也可用于其他病变，以确定HPV拷贝数和HPV类型，并且在确定用于治疗HPV相关病变的其他药物的抗病毒活性方面可能具有价值。
