Yang Y F, Tsao Y P, Yin C S, Chen S L, Chu T Y
Department of Obstetrics and Gynecology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
J Med Virol. 1996 Apr;48(4):302-7. doi: 10.1002/(SICI)1096-9071(199604)48:4<302::AID-JMV2>3.0.CO;2-B.
Infection with different types of human papillomavirus (HPV) is associated with neoplasia at different anatomic sites. The "low-risk" HPVs (LR-HPV) are responsible for benign genital lesions such as condyloma acuminata. In order to clarify the tumorigenic mechanism of LR-HPV, the HPV infection status was investigated and the expression of the c-jun proto-oncogene in different HPV-related skin and genital lesions analyzed. Of the 17 condyloma specimens analyzed by Western blotting, 13 cases (76.5%) exhibited overexpression of the c-jun gene. All 13 cases harbored high copy numbers of the LR-HPV genome with an average of 926 copies per cell, whereas the other four cases had an average of 12 copies of LR-HPV per cell (P < 0.001). Further typing of HPV by Southern blotting revealed that HPV-6 and HPV-11 infections predominated in c-jun positive cases. The c-jun protein was detected much less frequently in cervical cancers (three of 29, or 10.3%) and skin warts (one of 10), and was not detected in five genital polyps or in five normal cervical tissues. These findings suggest a type 6/11-specific induction of c-jun gene expression in HPV-related neoplastic lesions.
感染不同类型的人乳头瘤病毒（HPV）与不同解剖部位的肿瘤形成有关。“低风险”HPV（LR-HPV）可导致良性生殖器病变，如尖锐湿疣。为了阐明LR-HPV的致瘤机制，我们调查了HPV感染状况，并分析了不同HPV相关皮肤和生殖器病变中c-jun原癌基因的表达。在通过蛋白质印迹法分析的17份尖锐湿疣标本中，13例（76.5%）表现出c-jun基因的过表达。所有13例标本中LR-HPV基因组拷贝数均较高，平均每个细胞926个拷贝，而其他4例平均每个细胞有12个LR-HPV拷贝（P<0.001）。通过Southern印迹法对HPV进一步分型显示，HPV-6和HPV-11感染在c-jun阳性病例中占主导地位。在宫颈癌（29例中的3例，即10.3%）和皮肤疣（10例中的1例）中检测到c-jun蛋白的频率要低得多，在5例生殖器息肉和5例正常宫颈组织中未检测到c-jun蛋白。这些发现表明，在HPV相关肿瘤病变中存在6/11型特异性诱导c-jun基因表达的现象。
