Sakagami H, Sakagami T, Yoshida H, Omata T, Shiota F, Takahashi H, Kawazoe Y, Takeda M
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1995 May-Jun;15(3):917-21.
Chemiluminescence, generated by the mixture of sodium hypochlorite solution and luminol, was completely eliminated by polyphenols, such as natural lignins, phenylpropenoid monomers and polymers, and epigallocatechin gallate. On the other hand, hypochlorite scavenging activity of polysaccharides, such as PSK (Krestin) and Schizophyllan, was relatively weak. Human myelogenous leukemic cell lines (HL-60, ML-1) showed higher production of active oxygen(s) (detected by luminol chemiluminescence) and iodination capacity, than six other cultured cell lines. Since lignin did not completely eliminate the active oxygen production by HL-60 cells, possible stimulation of hypochlorite production by lignin was suggested.
次氯酸钠溶液与鲁米诺混合产生的化学发光被多酚类物质完全消除，如天然木质素、苯丙素类单体和聚合物以及表没食子儿没食子酸酯。另一方面，多糖类物质如PSK（云芝多糖）和裂褶多糖的次氯酸盐清除活性相对较弱。人骨髓性白血病细胞系（HL-60、ML-1）比其他六种培养细胞系表现出更高的活性氧产生量（通过鲁米诺化学发光检测）和碘化能力。由于木质素并未完全消除HL-60细胞产生的活性氧，因此提示木质素可能刺激次氯酸盐的产生。
