从植物病原真菌菜豆壳球孢中克隆出的一个独特的内切葡聚糖酶编码基因。
A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina.
作者信息
Wang H, Jones R W
机构信息
Department of Botany & Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Appl Environ Microbiol. 1995 May;61(5):2004-6. doi: 10.1128/aem.61.5.2004-2006.1995.
Abstract
The deduced amino acid sequence derived from a Macrophomina phaseolina beta-1,4-endoglucanase-encoding gene revealed 48% identity (over 119 amino acids) with egl1 from the phytopathogen Pseudomonas solanacearum. Its similarity to saprophyte endoglucanases was not significant. Its minimum substrate size, unlike that of any known saprophyte endoglucanase, was cellopentaose. The unique characteristics of M. phaseolina egl1-encoded endoglucanase suggest that it is phytopathogen specific.
摘要
从菜豆壳球孢β-1,4-内切葡聚糖酶编码基因推导的氨基酸序列显示，与植物病原菌青枯雷尔氏菌的egl1有48%的同一性（超过119个氨基酸）。它与腐生菌内切葡聚糖酶的相似性不显著。与任何已知腐生菌内切葡聚糖酶不同，它的最小底物大小是纤维五糖。菜豆壳球孢egl1编码的内切葡聚糖酶的独特特性表明它是植物病原菌特异性的。
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