感染马动脉炎病毒改良比塞洛斯毒株的BHK - 21细胞的超微结构和免疫细胞化学
Ultrastructure and immuno-cytochemistry of BHK-21 cells infected with a modified Bucyrus strain of equine arteritis virus.
作者信息
Wada R, Fukunaga Y, Kondo T, Kanemaru T
机构信息
Equine Research Institute, Japan Racing Association, Tochigi.
出版信息
Arch Virol. 1995;140(7):1173-80. doi: 10.1007/BF01322744.
Abstract
Morphogenesis of a modified Bucyrus strain of equine arteritis virus (EAV) in BHK-21 cells was studied. Bacillary tubules were first detected in the cytoplasm 8 h after infection, and mature virions 79 to 122 nm in diameter, 101 nm on average, were mostly observed in the cisternae of the rough endoplasmic reticulum (RER) at 12 h or later. They had isometrical cores and morphological subunits in the outer layer. Budding occurred from the RER and the outer nuclear membrane, but not from the cell surface. Structural linkage was detected between the tubule and the virus core. Aberrant strands were occasionally demonstrated within the nucleus 12 h after infection, and immunofluorescence and immunogold labeling revealed viral antigen also in the nucleus.
摘要
研究了马动脉炎病毒（EAV）改良比塞洛斯毒株在BHK-21细胞中的形态发生。感染后8小时在细胞质中首次检测到杆菌样小管，12小时及以后，大多在粗面内质网（RER）的潴泡中观察到直径79至122nm、平均101nm的成熟病毒粒子。它们具有等轴状核心和外层的形态亚基。出芽发生于RER和外核膜，但不发生于细胞表面。在小管与病毒核心之间检测到结构联系。感染后12小时偶尔在细胞核内可见异常链，免疫荧光和免疫金标记也显示细胞核内有病毒抗原。
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