Smadja N V, Louvet C, Isnard F, Dutel J L, Grange M J, Varette C, Krulik M
Laboratoire de Cytogénétique Hémato-Cancérologique, Hôpital Saint Antonie, Paris, France.
Br J Haematol. 1995 Jul;90(3):619-24. doi: 10.1111/j.1365-2141.1995.tb05593.x.
Cytogenetic studies in multiple myeloma (MM) have been disappointing due to the low mitotic index of plasma cells. Recently the detection of clonal chromosomal abnormalities at diagnosis seemed to be improved by addition of cytokines (IL-6 and GM-CSF) in the culture medium. We performed two parallel total bone marrow cells culture types in 33 stage I, II and III multiple myeloma patients at diagnosis: 3 d without any cytokine, and 4-7 days stimulated with IL-6 and GM-CSF. No clonal chromosomal abnormality was detected in the 12 stage I and II patients either in 3 d or in 4-7 d culture. In stage II patients, abnormalities were observed in 18/21 (85.7%) and in 8/18 (44.4%) in the 3 d culture and the 4-7 d stimulated cultures respectively. Our results suggest that in stage III multiple myeloma at diagnosis, 3 d culture without cytokine may be the better technique to detect clonal chromosomal abnormalities, and, before using cytokines as a reference condition, this 3 d unstimulated culture should be considered.
由于浆细胞的有丝分裂指数较低，多发性骨髓瘤（MM）的细胞遗传学研究一直不尽人意。最近，通过在培养基中添加细胞因子（IL-6和GM-CSF），诊断时克隆性染色体异常的检测似乎有所改善。我们对33例诊断为I、II和III期多发性骨髓瘤的患者进行了两种平行的全骨髓细胞培养：一种是在无任何细胞因子的情况下培养3天，另一种是用IL-6和GM-CSF刺激培养4 - 7天。在I期和II期的12例患者中，无论是在3天培养还是在4 - 7天培养中，均未检测到克隆性染色体异常。在II期患者中，3天培养和4 - 7天刺激培养中分别有18/21（85.7%）和8/18（44.4%）观察到异常。我们的结果表明，在诊断为III期的多发性骨髓瘤中，无细胞因子的3天培养可能是检测克隆性染色体异常的更好技术，并且在将细胞因子用作参考条件之前，应考虑这种3天未刺激的培养。
