Facon T, Lai J L, Nataf E, Preudhomme C, Zandecki M, Hammad M, Wattel E, Jouet J P, Bauters F
Service des Maladies du Sang, C.H.U., Lille, France.
Br J Haematol. 1993 Aug;84(4):743-5. doi: 10.1111/j.1365-2141.1993.tb03155.x.
Cytogenetic analysis was successfully performed in 46 consecutive myeloma patients (40 newly diagnosed and six relapsed patients). Karyotype was performed on bone marrow cells after long-term cultures (6 d) stimulated by GM-CSF, GM-CSF+IL6 or GM-CSF+IL6+IL3. Nineteen patients (41%) had cytogenetic abnormalities including 17/40 patients at diagnosis (42.5%) and 2/6 patients at relapse. Hyperdiploidy was found in 12 patients and hypodiploidy in four patients. Of the 17 newly diagnosed patients with cytogenetic abnormalities, five died from myeloma after 1-14 months and three other patients had primary drug resistance. Our results suggest that cytogenetic analysis after stimulation of cultures by cytokines detects clonal abnormalities in 40-50% of newly diagnosed myeloma patients and that these patients often have a short survival and/or primary drug resistance.
对46例连续的骨髓瘤患者（40例新诊断患者和6例复发患者）成功进行了细胞遗传学分析。在经GM-CSF、GM-CSF + IL6或GM-CSF + IL6 + IL3刺激的长期培养（6天）后，对骨髓细胞进行核型分析。19例患者（41%）存在细胞遗传学异常，其中诊断时17/40例患者（42.5%），复发时2/6例患者。12例患者发现超二倍体，4例患者发现亚二倍体。在17例新诊断的有细胞遗传学异常的患者中，5例在1 - 14个月后死于骨髓瘤，另外3例患者存在原发性耐药。我们的结果表明，细胞因子刺激培养后的细胞遗传学分析在40 - 50%的新诊断骨髓瘤患者中检测到克隆异常，并且这些患者通常生存期短和/或存在原发性耐药。
