Cuneo A, Balsamo R, Roberti M G, Bardi A, Piva N, Balboni M, Bigoni R, Rigolin G M, Castoldi G
Institute of Hematology, University of Ferrara, Italy.
Cancer Genet Cytogenet. 1996 Sep;90(2):171-5. doi: 10.1016/s0165-4608(96)00129-x.
To better define the role of interleukin-3 (IL-3) and IL-6 in the cytogenetic analysis of multiple myeloma (MM), we performed concomitant chromosome and cytologic studies in 34 patients. In each case, 10-30 x 10(6) bone marrow cells were incubated in two independent cultures consisting of conventional cytogenetic medium with and without IL-3 plus IL-6 added for 72 hours. 1-ml aliquots of each culture were aspirated at 24, 48, and 72 hours and exposed to colcemid for 6 hours. Cytospin preparations were then made and mitotic cells were counted and identified as plasma cells or as nonmalignant cells based on their reactivity with an appropriate anti kappa/lambda serum. Slides for conventional cytogenetic analysis were prepared at 72 hours. A greater than two-fold increase of mitotic plasma cells was observed in cytospin preparations from stimulated cultures versus unstimulated cultures in 15 of 34 cases, whereas a less than 2-fold increase, no variation or no mitosis was recorded in 19 cases. Comparison of the number of mitotic plasma cells in stimulated cultures at 24, 48, and 72 hours showed a decreased mitotic activity at 72 hours. Clonal abnormalities were detected by conventional cytogenetic analysis in 19 of 34 cases (55.8%). Recurrent clonal aberrations involved chromosome 13 (4 cases), chromosomes 1p, and 14q (3 cases); chromosomes 3p, 6q, 7q, and 9q (2 cases). We conclude that IL-3 + IL-6 may increase the number of dividing plasma cells in cytogenetic cultures and that a 2-day culture with these cytokines may facilitate the detection of chromosome abnormalities in MM.
为了更好地确定白细胞介素-3（IL-3）和白细胞介素-6在多发性骨髓瘤（MM）细胞遗传学分析中的作用，我们对34例患者进行了染色体和细胞学同步研究。在每例患者中，将10 - 30×10⁶个骨髓细胞在两种独立培养体系中培养，一种是添加了IL-3和IL-6的传统细胞遗传学培养基，另一种是未添加的，培养72小时。在24、48和72小时时，从每种培养体系中吸取1 ml等分试样，加入秋水仙酰胺处理6小时。然后制作细胞离心涂片，计数有丝分裂细胞，并根据其与合适的抗κ/λ血清的反应性将其鉴定为浆细胞或非恶性细胞。在72小时时制备用于传统细胞遗传学分析的玻片。在34例患者中，有15例患者的刺激培养体系的细胞离心涂片中观察到有丝分裂浆细胞数量比未刺激培养体系增加了两倍以上，而在19例患者中，有丝分裂浆细胞数量增加不到两倍、无变化或无有丝分裂。对24、48和72小时时刺激培养体系中有丝分裂浆细胞数量的比较显示，72小时时有丝分裂活性降低。通过传统细胞遗传学分析在34例患者中的19例（55.8%）检测到克隆性异常。常见的克隆性畸变涉及13号染色体（4例）、1p和14q染色体（3例）；3p、6q、7q和9q染色体（2例）。我们得出结论，IL-3 + IL-6可能会增加细胞遗传学培养中分裂浆细胞的数量，并且用这些细胞因子进行为期2天的培养可能有助于检测MM中的染色体异常。
