Landès C, Perona J J, Brunie S, Rould M A, Zelwer C, Steitz T A, Risler J L
Centre de Génétique Moléculaire, Université P & M Curie, Gif-sur-Yvette, France.
Biochimie. 1995;77(3):194-203. doi: 10.1016/0300-9084(96)88125-9.
The superimposable dinucleotide fold domains of MetRS, GlnRS and TyrRS define structurally equivalent amino acids which have been used to constrain the sequence alignments of the 10 class I aminoacyl-tRNA synthetases (aaRS). The conservation of those residues which have been shown to be critical in some aaRS enables to predict their location and function in the other synthetases, particularly: i) a conserved negatively-charged residue which binds the alpha-amino group of the amino acid substrate; ii) conserved residues within the inserted domain bridging the two halves of the dinucleotide-binding fold; and iii) conserved residues in the second half of the fold which bind the amino acid and ATP substrate. The alignments also indicate that the class I synthetases may be partitioned into two subgroups: a) MetRS, IleRS, LeuRS, ValRS, CysRS and ArgRS; b) GlnRS, GluRS, TyrRS and TrpRS.
甲硫氨酰 - tRNA合成酶（MetRS）、谷氨酰胺 - tRNA合成酶（GlnRS）和酪氨酸 - tRNA合成酶（TyrRS）的可叠加二核苷酸折叠结构域定义了结构上等效的氨基酸，这些氨基酸已被用于约束10种I类氨酰 - tRNA合成酶（aaRS）的序列比对。那些在某些aaRS中已被证明至关重要的残基的保守性，使得能够预测它们在其他合成酶中的位置和功能，特别是：i）一个结合氨基酸底物α - 氨基的保守带负电荷残基；ii）在连接二核苷酸结合折叠两部分的插入结构域内的保守残基；iii）在折叠后半部分结合氨基酸和ATP底物的保守残基。序列比对还表明，I类合成酶可分为两个亚组：a）MetRS、IleRS、LeuRS、ValRS、CysRS和ArgRS；b）GlnRS、GluRS、TyrRS和TrpRS。
