Nakata H, Itoh H, Yokoya Y, Kawai J, Nishioka S, Miyamoto K, Kitamoto N, Miyamoto H, Tanaka T
Second Department of Internal Medicine, Wakayama Medical College, Japan.
J Gastroenterol. 1995 Jun;30(3):295-300. doi: 10.1007/BF02347502.
We developed a highly specific detection technique for serum antibody, using a monoclonal antibody to a specific antigen of Helicobacter pylori. A monoclonal antibody preparation that reacted with the 54-kDa molecule of H. pylori antigens was obtained. Using this preparation, an antigen-capture enzyme-linked immunosorbent assay (ELISA) was established by fixation of the monoclonal antibody, followed by reaction with sonicated whole cell antigens. The serum antibody titers of patients with gastritis and peptic ulcers were significantly higher than control titers, and the antibody titer correlated with the histological severity of gastritis. Patients positive for H. pylori by bacterial culture had higher titers than H. pylori-negative patients. Our new ELISA may be useful for the diagnosis of H. pylori infections and for evaluation of the severity of gastric inflammation.
我们利用针对幽门螺杆菌特定抗原的单克隆抗体，开发了一种高度特异性的血清抗体检测技术。获得了一种与幽门螺杆菌抗原的54-kDa分子发生反应的单克隆抗体制剂。使用该制剂，通过固定单克隆抗体，随后与超声处理的全细胞抗原反应，建立了一种抗原捕获酶联免疫吸附测定（ELISA）。胃炎和消化性溃疡患者的血清抗体滴度显著高于对照滴度，且抗体滴度与胃炎的组织学严重程度相关。经细菌培养幽门螺杆菌呈阳性的患者滴度高于幽门螺杆菌阴性患者。我们新的ELISA可能有助于幽门螺杆菌感染的诊断以及评估胃炎症的严重程度。
