Tamaoka A, Sawamura N, Odaka A, Suzuki N, Mizusawa H, Shoji S, Mori H
Department of Neurology, University of Tsukuba, Japan.
Brain Res. 1995 May 8;679(1):151-6. doi: 10.1016/0006-8993(95)00162-j.
Diffuse plaques are immature and amorphous senile plaques and believed to be in the initial phase of plaque formation. In contrast to amyloid angiopathy and the plaque core amyloid, diffuse plaques failed to be purified in preserved forms from the brain. Here, we studied the diffuse plaques in the cerebellar region of the Alzheimer's disease brain based on immunocytochemistry and ELISA using two different monoclonal antibodies specifically recognizing the carboxyl termini of A beta molecules (BA27 for A beta 1-40 and BC05 for A beta 1-42/43). We found that the amount of A beta 1-40 was in proportion to the staining degree on amyloid angiopathy by immunohistochemistry. We found that A beta 1-42/43 comprised diffuse plaques as the major component in the cerebella of AD brains. Taking these findings into consideration, diffuse plaques, the earliest pathological change in the brain with AD, are concluded to be composed mainly of A beta 1-42/43, implicating the critical importance of this kind of A beta species deposition in the pathogenesis of AD.
弥漫性斑块是不成熟的无定形老年斑，被认为处于斑块形成的初始阶段。与淀粉样血管病和斑块核心淀粉样蛋白不同，弥漫性斑块无法以保存的形式从大脑中纯化出来。在此，我们基于免疫细胞化学和酶联免疫吸附测定（ELISA），使用两种特异性识别Aβ分子羧基末端的不同单克隆抗体（用于Aβ1-40的BA27和用于Aβ1-42/43的BC05），研究了阿尔茨海默病大脑小脑区域的弥漫性斑块。我们发现，通过免疫组织化学，Aβ1-40的量与淀粉样血管病的染色程度成比例。我们发现，Aβ1-42/43在AD大脑的小脑中构成弥漫性斑块的主要成分。考虑到这些发现，弥漫性斑块作为AD大脑中最早的病理变化，被认为主要由Aβ1-42/43组成，这表明这种Aβ种类的沉积在AD发病机制中至关重要。
