Jester J V, Petroll W M, Barry P A, Cavanagh H D
Department of Ophthalmology, University of Texas, Southwestern Medical Center at Dallas, USA.
J Anat. 1995 Apr;186 ( Pt 2)(Pt 2):301-11.
We have evaluated temporally the 3-dimensional cellular anatomy of corneal wound tissue in the rabbit eye using in vivo tandem scanning confocal microscopy. In vivo microscopic studies showed that corneal fibroblast migrated into the wound as an interconnected cellular meshwork with long, thin, randomly oriented cell processes. Interconnection of fibroblasts was further confirmed by localisation of monoclonal antibodies to connexin 43 which demonstrated prominent staining of putative gap junctions between fibroblasts. Temporal observations indicated that the interconnected cells and cellular processes undergo sequential positional changes leading to orientation of cells and interconnected cell processes parallel to the wound margin. Laser scanning confocal microscopy of en bloc, phalloidin-stained corneal wounds showed prominent intracellular f-actin bundles (i.e. stress fibres) within cell processes which formed an extensive interwoven pattern within the wound.
我们使用体内串联扫描共聚焦显微镜对兔眼角膜伤口组织的三维细胞解剖结构进行了时间评估。体内显微镜研究表明，角膜成纤维细胞以相互连接的细胞网络形式迁移到伤口中，这些细胞网络具有长而细、随机取向的细胞突起。通过针对连接蛋白43的单克隆抗体定位进一步证实了成纤维细胞的相互连接，该定位显示了成纤维细胞之间假定的间隙连接的显著染色。时间观察表明，相互连接的细胞和细胞突起会经历连续的位置变化，导致细胞和相互连接的细胞突起平行于伤口边缘排列。对整块用鬼笔环肽染色的角膜伤口进行激光扫描共聚焦显微镜检查，结果显示细胞突起内有突出的细胞内f-肌动蛋白束（即应力纤维），这些应力纤维在伤口内形成了广泛的交织模式。
