Lynch N R, Dunand P, Newcomb R W, Chai H, Bigley J
Clin Exp Immunol. 1975 Oct;22(1):35-46.
The radioallergosorbent test (RAST) for alternaria was compared to skin tests and bronchial challenges in children suffering from chronic intractable asthma. In contrast to when such children were tested with a timothy grass pollen extract, the bronchial challenge and skin test results against alternaria did not correlate significantly. When alternaria allergens were coupled to cyanogen bromide-activated microcrystalline cellulose, the RAST correlated with the results of skin testing but not bronchial challenge. It was demonstrated by column immunabsorption that some allergic sera contained sufficient IgG antibody against alternaria to competitively inhibit the RAST. When Sepharose 2B was substituted for cellulose as the insoluble support, the inhibition by IgG antibody was largely overcome and then the RAST correlated with both skin test and bronchial challenge results. Glycopeptides contribute significantly to the allergenicity of alternaria, and when these materials were coupled to a Sepharose 2B conjugate by mild oxidation, the RAST correlated with bronchial challenge, but not skin test, results. It was concluded that in this group of steroid-dependent asthmatic children, the correlation of the RAST with the in vivo challenges was strongly influenced by the presence of IgG antibody in the allergic sera and the chemical nature of the mould allergens investigated.
对患有慢性难治性哮喘的儿童，进行了针对链格孢属的放射变应原吸附试验（RAST），并与皮肤试验和支气管激发试验进行比较。与用梯牧草花粉提取物对这类儿童进行检测时不同，针对链格孢属的支气管激发试验和皮肤试验结果没有显著相关性。当链格孢属变应原与溴化氰活化的微晶纤维素偶联时，RAST与皮肤试验结果相关，但与支气管激发试验结果不相关。柱免疫吸附表明，一些过敏血清含有足够的抗链格孢属IgG抗体来竞争性抑制RAST。当用琼脂糖2B替代纤维素作为不溶性支持物时，IgG抗体的抑制作用在很大程度上被克服，此时RAST与皮肤试验和支气管激发试验结果均相关。糖肽对链格孢属的变应原性有显著贡献，当通过轻度氧化将这些物质与琼脂糖2B偶联物结合时，RAST与支气管激发试验结果相关，但与皮肤试验结果不相关。得出的结论是，在这组依赖类固醇的哮喘儿童中，RAST与体内激发试验的相关性受过敏血清中IgG抗体的存在以及所研究的霉菌变应原的化学性质的强烈影响。
