编码核糖核酸酶II的大肠杆菌rnb基因的精确物理图谱。
Precise physical mapping of the Escherichia coli rnb gene, encoding ribonuclease II.
作者信息
Zilhão R, Caillet J, Régnier P, Arraiano C M
机构信息
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
出版信息
Mol Gen Genet. 1995 Jul 28;248(2):242-6. doi: 10.1007/BF02190807.
PMID:7651348
Abstract
Ribonuclease II (encoded by rnb) is one of the two main exonucleases involved in mRNA degradation in Escherichia coli. We report the precise physical mapping of rnb to 29 min on the chromosomal map in the vicinity of pyrF, and clarify the genetic and physical maps of this E. coli chromosomal region. The results were confirmed by the construction of a strain partially deleted for rnb.
摘要
核糖核酸酶II（由rnb编码）是参与大肠杆菌mRNA降解的两种主要核酸外切酶之一。我们报告了rnb在染色体图谱上精确的物理定位，位于pyrF附近的29分钟处，并阐明了该大肠杆菌染色体区域的遗传图谱和物理图谱。通过构建一个rnb部分缺失的菌株，证实了这些结果。
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