Valen G, Kaszaki J, Szabó I, Nagy S, Vaage J
Department of Surgery and Anesthesiology, University of Tromsø, Norway.
J Mol Cell Cardiol. 1993 Jan;25(1):31-40. doi: 10.1006/jmcc.1993.1005.
Histamine is synthetized in the heart, and released by ischemia-reperfusion injury in several species. Histamine has arrhythmogenic, chronotropic, inotropic and vasoactive effects. Cardiac histamine release during ischemia-reperfusion may be mediated by toxic oxygen metabolites. We studied the effect of ischemia-reperfusion and toxic oxygen metabolites on release and synthesis of histamine in the isolated rat heart (Langendorff model). The following groups were included: I, (n = 10) control perfusion for 60 min; II, (n = 7) H2O2 (200 microM) was given for 10 min followed by 50 min recovery; III, (n = 7) thiourea (15 mM) was given in addition to H2O2; IV, (n = 7) thiourea given alone; V, (n = 7) catalase (150 U/ml) plus H2O2; VI, (n = 7) 20 min ischemia followed by 40 min reperfusion. The contents of histamine in the coronary effluent and in cardiac tissue were measured repeatedly (radioenzymatic method). Ischemia-reperfusion and toxic oxygen metabolites increased release of histamine in the coronary effluent. Concomitantly the histamine contents in cardiac tissue increased, indicating increased synthesis of histamine.
组胺在心脏中合成，并在几种物种中由缺血再灌注损伤释放。组胺具有致心律失常、变时性、变力性和血管活性作用。缺血再灌注期间心脏组胺的释放可能由有毒氧代谢产物介导。我们研究了缺血再灌注和有毒氧代谢产物对离体大鼠心脏（Langendorff模型）中组胺释放和合成的影响。包括以下几组：I组（n = 10），进行60分钟的对照灌注；II组（n = 7），给予过氧化氢（200微摩尔）10分钟，然后恢复50分钟；III组（n = 7），除过氧化氢外还给予硫脲（15毫摩尔）；IV组（n = 7），单独给予硫脲；V组（n = 7），过氧化氢酶（150单位/毫升）加过氧化氢；VI组（n = 7），缺血20分钟，然后再灌注40分钟。反复测量冠状动脉流出液和心脏组织中的组胺含量（放射酶法）。缺血再灌注和有毒氧代谢产物增加了冠状动脉流出液中组胺的释放。与此同时，心脏组织中的组胺含量增加，表明组胺合成增加。
