Salyer K E, Bardach J, Squier C A, Gendler E, Kelly K M
Research Laboratory of the International Craniofacial Institute, Medical City Dallas Hospital, Texas, USA.
Plast Reconstr Surg. 1995 Sep;96(4):770-9. doi: 10.1097/00006534-199509001-00002.
This study was designed to test the hypothesis that demineralized perforated bone matrix implant from canine skull and tibia induces new bone formation within the calvarial defect comparable with the bone induced by autogenous graft. We also were interested in determining whether demineralized perforated bone matrix implants from membranous bone have greater osseoinductive capacity in the calvarial area than demineralized perforated bone matrix implants from endochondral bone. Forty 12-week-old purebred beagles were used. Group I consisted of animals with unrepaired surgically created calvarial defects healed by secondary intention (n = 10). Group II consisted of animals with surgically created calvarial defects in which the bone was removed and replaced with an autograft (n = 10). Group III consisted of animals with surgically created calvarial defects in which the bony defect was closed with a demineralized perforated bone matrix implant obtained from beagle calvaria (n = 10). Group IV consisted of animals with surgically created calvarial defects in which the bony defect was closed with a demineralized perforated bone matrix implant obtained from beagle tibia (n = 10). The two control groups (I and II) allowed us to isolate the inductive capacity of demineralized perforated bone matrix implants and compare it with the healing of the bone defects left unrepaired or repaired with calvarial autografts. Animals were sacrificed after 8 and 12 weeks. In the present study we were able to verify that demineralized perforated bone matrix implants are well accepted in the calvarial defects with little tissue reaction and remarkably little osteoclastic activity.(ABSTRACT TRUNCATED AT 250 WORDS)
犬颅骨和胫骨的脱矿穿孔骨基质植入物在颅骨缺损处诱导新骨形成的能力与自体移植物诱导的骨形成能力相当。我们还想确定，来自膜性骨的脱矿穿孔骨基质植入物在颅骨区域的骨诱导能力是否比来自软骨内骨的脱矿穿孔骨基质植入物更强。使用了40只12周龄的纯种比格犬。第一组由手术造成颅骨缺损后通过二期愈合的动物组成（n = 10）。第二组由手术造成颅骨缺损后去除骨并用自体移植物替代的动物组成（n = 10）。第三组由手术造成颅骨缺损后用从比格犬颅骨获得的脱矿穿孔骨基质植入物封闭骨缺损的动物组成（n = 10）。第四组由手术造成颅骨缺损后用从比格犬胫骨获得的脱矿穿孔骨基质植入物封闭骨缺损的动物组成（n = 10）。两个对照组（第一组和第二组）使我们能够分离脱矿穿孔骨基质植入物的诱导能力，并将其与未修复或用颅骨自体移植物修复的骨缺损的愈合情况进行比较。8周和12周后处死动物。在本研究中，我们能够证实脱矿穿孔骨基质植入物在颅骨缺损中被很好地接受，组织反应小，破骨细胞活性显著低。（摘要截短于250字）
