Togawa K, Rustgi A K
Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Virus Res. 1995 May;36(2-3):293-7. doi: 10.1016/0168-1702(95)00011-e.
Over 65 human papillomaviruses (HPVs) have been identified. The majority have been isolated on the basis of cloning systems in bacteria. Recently, general consensus or degenerate primers from the L1 region have been used in PCR to identify novel genotypes. In this fashion, we employed general primers in the L1 region followed by DNA sequencing to identify a novel HPV sequence in association with esophageal squamous cell carcinoma.
已鉴定出65种以上的人乳头瘤病毒（HPV）。大多数是基于细菌中的克隆系统分离出来的。最近，来自L1区域的通用引物或简并引物已用于聚合酶链反应（PCR）以鉴定新的基因型。通过这种方式，我们使用L1区域的通用引物，然后进行DNA测序，以鉴定与食管鳞状细胞癌相关的一种新的HPV序列。
