耐镉金黄色葡萄球菌17810R中Cd2+外排系统的能量学
Energetics of Cd2+ efflux system in cadmium-resistant Staphylococcus aureus 17810R.
作者信息
Tynecka Z, Malm A
机构信息
Department of Pharmaceutical Microbiology, Medical Academy, Lublin, Poland.
出版信息
Acta Biochim Pol. 1995;42(1):119-23.
Energetics of 109Cd efflux in resting cells of cadmium-resistant Staphylococcus aureus 17810R was assayed in 1 or 100 mM potassium/sodium phosphate buffer, pH7 (PB). Experiments with the use of inhibitors and ionophores showed that Cd2+ extrusion in this organism required ATP and either a pH gradient (delta pH) in 1 mM PB or membrane potential (delta psi) in 100 mM PB. The role of high phosphate ion concentration in delta psi-dependent Cd2+ efflux is discussed.
在pH7的1 mM或100 mM磷酸钾/钠缓冲液（PB）中测定了耐镉金黄色葡萄球菌17810R静息细胞中109Cd流出的能量学。使用抑制剂和离子载体的实验表明，该生物体中Cd2+的外排需要ATP以及在1 mM PB中的pH梯度（ΔpH）或在100 mM PB中的膜电位（Δψ）。讨论了高磷酸根离子浓度在依赖Δψ的Cd2+外排中的作用。
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