Imayama S, Shimozono Y, Urabe A, Hori Y
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Acta Derm Venereol. 1995 May;75(3):212-7. doi: 10.2340/0001555575212217.
We developed a simple method for measuring the amount of the secretory form of immunoglobulin A (sIgA) present in sweat. A small disk (10 x 10 mm) made of cellulose membrane was attached to the skin surface for periods of 1 to 24 h. SIgA was absorbed to the membrane and accumulated during the period of application. Enzyme immunoassay using anti-sIgA and antisecretory component (SC) antibodies revealed distinct dots on the disk that corresponded to the eccrine excretory ducts. A densitograph was used to determine the number and density of the dots, thus obtaining the amount of sIgA excreted to the surface of the skin (per mm2). The amount of skin sIgA excreted differed inter-individually as well as intra-individually. That is, it varied according to the region of the skin, and its distribution roughly reflected that of the sweat ducts. SIgA excretion was maintained at a certain level, regardless of the increased sweating produced by either heat or exercise, which raised the output of sweat 3- to 15-fold. Immunohistochemical studies revealed that fewer glandular cells expressed SC in their cytoplasm as the amount of sIgA decreased. Such an independence of the excretion of sIgA from that of sweat may be necessary to the local immune defenses of the skin.
我们开发了一种简单的方法来测量汗液中分泌型免疫球蛋白A（sIgA）的含量。将一片由纤维素膜制成的小圆盘（10×10毫米）贴在皮肤表面1至24小时。在此期间，sIgA被吸收到膜上并积聚。使用抗sIgA和抗分泌成分（SC）抗体的酶免疫测定法显示圆盘上出现与外分泌排泄管相对应的明显斑点。使用密度计确定斑点的数量和密度，从而得出排泄到皮肤表面的sIgA量（每平方毫米）。皮肤sIgA的排泄量在个体间和个体内均存在差异。也就是说，它会因皮肤区域而异，其分布大致反映了汗腺导管的分布。无论热或运动导致出汗增加（使汗液分泌量增加3至15倍），sIgA的排泄都维持在一定水平。免疫组织化学研究表明，随着sIgA量的减少，腺细胞胞质中表达SC的细胞数量也减少。sIgA排泄与汗液排泄的这种独立性可能对皮肤的局部免疫防御至关重要。
