Didierjean L, Gruaz D, Frobert Y, Grassi J, Dayer J M, Saurat J H
Department of Medicine, Hôpital Cantonal Universitaire, Genève, Switzerland.
Cytokine. 1990 Nov;2(6):438-46. doi: 10.1016/1043-4666(90)90053-v.
Human eccrine sweat devoid of epidermal protein contamination was collected from palms, soles, and different sites on the trunk. Interleukin 1 alpha (IL 1 alpha) and interleukin 1 beta (IL 1 beta) content were analyzed for immunoreactivity by enzymo-immunoassay and immunoblotting and for bioactivity by the stimulation of prostaglandin E2 (PGE2) production in human dermal fibroblasts. The bioassay was validated by using blocking antibodies against IL 1 alpha and beta. All sweat samples were found to contain significant amounts of immunoreactive and biologically active IL 1. The immunoreactive forms were at 17 kDa as shown by immunoblotting analysis, indicating that they were mature (secreted), undegraded IL 1 peptides. Whereas IL 1 alpha was detectable in sweat samples obtained from both truncal and palmo-plantar regions, IL 1 beta was only detectable in the sweat of palms and soles (IL 1 alpha/beta ratio greater than 700 in trunk and 5.4 in palms and soles) indicating a site-dependent difference in the excretion of the two IL 1 molecules. IL 1 concentration was high in spontaneous (IL 1 alpha, 3.7; IL 1 beta, 0.3 ng/mL) and pilocarpine induced sweat (IL 1 alpha, 3.9; IL 1 beta, 1.2 ng/mL), and it was much increased during jogging and sauna (IL 1 alpha, 22.6; IL 1 beta, 3.3 ng/mL). This does not appear to represent an excretory process aimed at clearing blood IL 1, but rather a stress-induced increased production of IL 1 by sweat gland cells.
从手掌、脚底以及躯干的不同部位收集未受表皮蛋白污染的人外泌汗腺汗液。采用酶免疫测定法和免疫印迹法分析白细胞介素1α（IL - 1α）和白细胞介素1β（IL - 1β）的免疫反应性含量，并通过刺激人皮肤成纤维细胞中前列腺素E2（PGE2）的产生来分析其生物活性。通过使用针对IL - 1α和β的阻断抗体对生物测定进行验证。发现所有汗液样本均含有大量具有免疫反应性和生物活性的IL - 1。免疫印迹分析显示，免疫反应性形式的分子量为17 kDa，表明它们是成熟的（分泌型）、未降解的IL - 1肽。虽然在从躯干以及掌跖区域获得的汗液样本中均可检测到IL - 1α，但IL - 1β仅在手掌和脚底的汗液中可检测到（躯干中IL - 1α/β比值大于700，手掌和脚底中为5.4），这表明两种IL - 1分子的排泄存在部位依赖性差异。IL - 1浓度在自发性汗液（IL - 1α，3.7；IL - 1β，0.3 ng/mL）和毛果芸香碱诱导的汗液（IL - 1α，3.9；IL - 1β，1.2 ng/mL）中较高，在慢跑和桑拿期间显著增加（IL - 1α，22.6；IL - 1β，3.3 ng/mL）。这似乎并不代表旨在清除血液中IL - 1的排泄过程，而是应激诱导汗腺细胞IL - 1产生增加的过程。
