King W W, Lam P K, Li A K
Department of Surgery, Prince of Wales Hospital, Chinese University of Hong Kong.
Acta Otolaryngol. 1995 May;115(3):455-8. doi: 10.3109/00016489509139347.
Flow cytometric analysis of nuclear DNA content was performed in 34 squamous cell carcinomas of oral cavity and base of tongue tumours using archived paraffin-embedded tissues. The DNA content was correlated with the presence of cervical metastasis. Ten and 24 patients were classified as early (I & II) and advanced (III & IV) clinical stages, respectively. The DNA index (DI) was grouped into diploid (DI 0.85-1.15) and non-diploid. Seven (70%) tumours were non-diploid in clinical stages I & II. Four out of 7 (57%) developed initial and late cervical lymph node metastasis (p > 0.05). There were 15 (62.5%) non-diploid tumours in clinical stages III & IV. Thirteen out of 15 (86.7%) had cervical lymph node metastases (p < 0.05). However, the survival period and the incidence of recurrent disease for the whole group did not show any association with DNA ploidy. Our results suggest that DNA content may be useful as a reliable predictor of regional metastasis in advanced stage carcinoma of the tongue.
利用存档的石蜡包埋组织，对34例口腔和舌根鳞状细胞癌肿瘤进行了细胞核DNA含量的流式细胞术分析。DNA含量与颈部转移的存在相关。分别有10例和24例患者被归类为早期（I和II期）和晚期（III和IV期）临床阶段。DNA指数（DI）分为二倍体（DI 0.85 - 1.15）和非二倍体。在I和II期临床阶段，7例（70%）肿瘤为非二倍体。7例中的4例（57%）发生了早期和晚期颈部淋巴结转移（p>0.05）。在III和IV期临床阶段，有15例（62.5%）非二倍体肿瘤。15例中的13例（86.7%）有颈部淋巴结转移（p<0.05）。然而，整个组的生存期和复发疾病的发生率与DNA倍体没有任何关联。我们的结果表明，DNA含量可能作为晚期舌癌区域转移的可靠预测指标。
