Mahmood J U, Suzuki K, Nomura T, Shingaki S, Nakajima T
First Department of Oral and Maxillofacial Surgery, School of Dentistry, Niigata University, Japan.
Int J Oral Maxillofac Surg. 1995 Dec;24(6):427-32. doi: 10.1016/s0901-5027(05)80472-2.
Flow cytometry DNA analysis was performed on fresh tissue samples of 90 primary lesions and 32 metastatic lymph nodes of squamous cell carcinomas of the oral cavity and related regions to elucidate the characteristics of tumors with metastatic potency. The incidence of aneuploidy for carcinomas with metastasis was 67%, which was significantly higher than the 44% of carcinomas without metastasis, and aneuploid carcinomas (39%) had a higher tendency to metastasis than diploid carcinomas (20%). The incidence of aneuploidy and metastasis was related to the T classification, the degree of differentiation, and the histologic grade of malignancy. The incidence of aneuploidy and mean DNA index of metastatic lesions were 31% and 1.12, respectively, and the values were significantly lower than the 67% and 1.30 of the corresponding primary lesions. The results indicate that the chance of evolution of metastatic cell lines is higher in aneuploid carcinomas than diploid carcinomas, possible because the former is more heterogeneous than the latter, but most of the cell lines causing lymph-node metastasis are diploid cell lines. Metastatic lesions had a lower S-phase fraction than primary lesions, indicating that a high S-phase fraction does not always reflect the presence of metastatic cell lines.
对90例口腔及相关区域鳞状细胞癌原发灶和32例转移淋巴结的新鲜组织样本进行流式细胞术DNA分析，以阐明具有转移潜能肿瘤的特征。有转移的癌非整倍体发生率为67%，显著高于无转移癌的44%，且非整倍体癌（39%）比二倍体癌（20%）有更高的转移倾向。非整倍体和转移的发生率与T分类、分化程度及组织学恶性分级有关。转移灶的非整倍体发生率和平均DNA指数分别为31%和1.12，这些值显著低于相应原发灶的67%和1.30。结果表明，非整倍体癌比二倍体癌产生转移细胞系的几率更高，可能是因为前者比后者更具异质性，但大多数导致淋巴结转移的细胞系是二倍体细胞系。转移灶的S期分数低于原发灶，表明高S期分数并不总是反映转移细胞系的存在。
