Lim F, Cooper S L
Department of Chemical Engineering, University of Wisconsin-Madison 53706, USA.
Biomaterials. 1995 Apr;16(6):457-66. doi: 10.1016/0142-9612(95)98818-y.
A series of poly(tetramethylene oxide)-based polyurethane block copolymers was synthesized with varying levels of sulphonate ion incorporated in the hard segment block. Static in vitro adhesion of polymorphonuclear leucocytes (PMNs) on the underivatized and propyl sulphonate-grafted polyurethanes was investigated. The effect of plasma proteins on PMN adhesion to the polymers was also examined. The number of adherent cells on the sulphonated polyurethane surfaces was significantly higher than on the underivatized polyurethane under all conditions. The adherent PMNs on sulphonated polyurethanes showed increased cell spreading compared to the underivatized base polyurethane. Increasing the level of sulphonate incorporation from 5 to 17 mol% had no effect on the extent of cell adhesion. The presence of preadsorbed proteins generally led to a decrease in the number of adherent PMNs on the surfaces. However, the presence of plasma proteins in the suspension medium had no effect on the degree of leucocyte adhesion to the sulphonated polyurethanes.
合成了一系列基于聚四亚甲基醚的聚氨酯嵌段共聚物，其中硬段嵌段中引入了不同含量的磺酸根离子。研究了多形核白细胞（PMN）在未衍生化和丙基磺酸酯接枝的聚氨酯上的静态体外粘附情况。还考察了血浆蛋白对PMN与聚合物粘附的影响。在所有条件下，磺化聚氨酯表面的粘附细胞数量均显著高于未衍生化聚氨酯表面。与未衍生化的基础聚氨酯相比，磺化聚氨酯上粘附的PMN细胞铺展增加。将磺酸根掺入量从5 mol%提高到17 mol%对细胞粘附程度没有影响。预吸附蛋白质的存在通常会导致表面粘附的PMN数量减少。然而，悬浮介质中血浆蛋白的存在对白细胞与磺化聚氨酯的粘附程度没有影响。
