Düring K
University of Hamburg, Center for Applied Plant Molecular Biology, Germany.
Transgenic Res. 1994 Mar;3(2):138-40. doi: 10.1007/BF01974093.
Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during field testing of the transgenic plants or even more in the case of commercialization. The aim of this study was to produce a plant transformation vector which only contains a selectable and screenable marker gene and a multiple cloning site for insertion of promoter::foreign gene::terminator cassettes from other plasmids.
通过根癌农杆菌进行植物转化通常使用双元载体。大多数现有的双元载体在T-DNA（转移到植物基因组中的部分）中包含预期修饰不需要的元件。这些额外的序列在转基因植物的田间试验期间可能会带来潜在风险，在商业化情况下甚至风险更大。本研究的目的是构建一种植物转化载体，该载体仅包含一个可选择和可筛选的标记基因以及一个多克隆位点，用于插入来自其他质粒的启动子::外源基因::终止子盒。
